Assessment of thermally stabilized electrospun poly(vinyl alcohol) materials as cell permeable membranes for a novel blood salvage device.

Autor: Homer WJA; Engineering for Health Research Centre, College of Engineering and Physical Sciences, Aston University, Birmingham, UK., Lisnenko M; Dpt. Of Chemistry, Faculty of Science, Humanities and Education, Technical University of Liberec, Liberec, Czech Republic., Gardner AC; The Royal Orthopaedic Hospital NHS Foundation Trust, Birmingham, UK; College of Health and Life Sciences, Aston University, Birmingham, UK., Kostakova EK; Dpt. Of Chemistry, Faculty of Science, Humanities and Education, Technical University of Liberec, Liberec, Czech Republic., Valtera J; Dpt. Of Textile Machine Design, Faculty of Mechanical Engineering, Technical University of Liberec, Liberec, Czech Republic., Wall IB; College of Health and Life Sciences, Aston University, Birmingham, UK., Jencova V; Dpt. Of Chemistry, Faculty of Science, Humanities and Education, Technical University of Liberec, Liberec, Czech Republic., Topham PD; Chemical Engineering and Applied Chemistry, College of Engineering and Physical Sciences, Aston University, Birmingham, UK., Theodosiou E; Engineering for Health Research Centre, College of Engineering and Physical Sciences, Aston University, Birmingham, UK. Electronic address: e.theodosiou@aston.ac.uk.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2023 Jan; Vol. 144, pp. 213197. Date of Electronic Publication: 2022 Nov 17.
DOI: 10.1016/j.bioadv.2022.213197
Abstrakt: The use of Intraoperative Cell Salvage (ICS) is currently limited in oncological surgeries, due to safety concerns associated with the ability of existing devices to successfully remove circulating tumour cells. In this work, we present the first stages towards the creation of an alternative platform to current cell savers, based on the extremely selective immunoaffinity membrane chromatography principle. Non-woven membranes were produced via electrospinning using poly(vinyl alcohol) (PVA), and further heat treated at 180 °C to prevent their dissolution in aqueous environments and preserve their fibrous morphology. The effects of the PVA degree of hydrolysis (DH) (98 % vs 99 %), method of electrospinning (needleless DC vs AC), and heat treatment duration (1-8 h) were investigated. All heat treated supports maintained their cytocompatibility, whilst tensile tests indicated that the 99 % hydrolysed DC electrospun mats were stronger compared to their 98 % DH counterparts. Although, and at the described conditions, AC electrospinning produced fibres with more than double the diameter compared to those from DC electrospinning, it was not chosen for subsequent experiments because it is still under development. Evidence of unimpeded passage of SY5Y neuroblastoma cells and undiluted defibrinated sheep's blood in flow-through filtration experiments confirmed the successful creation of 3D networks with minimum resistance to mass transfer and lack of non-specific cell binding to the base material, paving the way for the development of novel, highly selective ICS devices for tumour surgeries.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Eirini Theodosiou reports financial support was provided by The Royal Society. Eirini Theodosiou reports financial support was provided by The Birmingham Orthopaedic Charity. Vera Jencova reports financial support was provided by The Czech Health Research Council.
(Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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