Validation of analytical method of cannabinoids: Novel approach using turbo-extraction.

Autor: Mano-Sousa BJ; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil. Electronic address: brayanmano@live.com., Alves BC; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil., Pedrosa AM; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil., Lima PL; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil; Posto de Perícia Integrado, 7° Departamento de Polícia Civil de Minas Gerais (PCMG), Divinópolis, Minas Gerais, Brazil., Andrade FP; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil., Duarte-Almeida JM; Universidade Federal de São João Del-Rei, Campus Centro-Oeste, Minas Gerais, Brazil. Electronic address: maudall@ufsj.edu.br.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 Mar 01; Vol. 254, pp. 124108. Date of Electronic Publication: 2022 Nov 17.
DOI: 10.1016/j.talanta.2022.124108
Abstrakt: The development of simple, efficient, and low-cost analytical methods is essential for the evaluation and monitoring of the main cannabinoids in Cannabis-based products. In this sense, the objectives of this study were to develop and validate an analytical method for obtaining and determining cannabinoids in a pool sample. Two extraction techniques were used, ultrasound and turbo-extraction, and two system-solvents, methanol:chloroform (9:1 v:v) and ethanol. The analytical method used and validated was carried out in High Performance Liquid Chromatography with Diodes Array Detector. The cannabidiol standard was characterized by a nuclear magnetic resonance. The use of the proposed method makes it possible to identify cannabinoids, both in the acid form and in the neutral form, in 7 min of analysis. The results confirmed high precision and accuracy. The detection and quantification limits were 0.19 μg/mL and 5 μg/mL, respectively. The method developed proved to be selective and robust for the evaluation of cannabinoids. It is hoped that the methods developed can be used to obtain and analyze cannabinoids, both for medicinal purposes and for forensic analysis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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