Intrapericardial cardiosphere-derived cells hinder epicardial dense scar expansion and promote electrical homogeneity in a porcine post-infarction model.

Autor:	Carta-Bergaz A; Gregorio Marañón Health Research Institute (IiSGM), Department of Cardiology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain., Ríos-Muñoz GR; Gregorio Marañón Health Research Institute (IiSGM), Department of Cardiology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.; Department of Bioengineering and Space Engineering, Universidad Carlos III de Madrid, Madrid, Spain., Crisóstomo V; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.; Jesús Usón Minimally Invasive Surgery Centre, Cáceres, Spain., Sánchez-Margallo FM; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.; Jesús Usón Minimally Invasive Surgery Centre, Cáceres, Spain., Ledesma-Carbayo MJ; Biomedical Image Technologies, ETSI Telecomunicación, Universidad Politécnica de Madrid, Madrid, Spain.; CIBER-BBN, Instituto de Salud Carlos III, Madrid, Spain., Bermejo-Thomas J; Gregorio Marañón Health Research Institute (IiSGM), Department of Cardiology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.; Medical School, Universidad Complutense de Madrid, Madrid, Spain., Fernández-Avilés F; Gregorio Marañón Health Research Institute (IiSGM), Department of Cardiology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.; Medical School, Universidad Complutense de Madrid, Madrid, Spain., Arenal-Maíz Á; Gregorio Marañón Health Research Institute (IiSGM), Department of Cardiology, Hospital General Universitario Gregorio Marañón, Madrid, Spain.; Centre for Biomedical Research in Cardiovascular Disease Network (CIBERCV), Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Frontiers in physiology [Front Physiol] 2022 Nov 15; Vol. 13, pp. 1041348. Date of Electronic Publication: 2022 Nov 15 (Print Publication: 2022).
DOI:	10.3389/fphys.2022.1041348
Abstrakt:	The arrhythmic substrate of ventricular tachycardias in many structural heart diseases is located in the epicardium, often resulting in poor outcomes with currently available therapies. Cardiosphere-derived cells (CDCs) have been shown to modify myocardial scarring. A total of 19 Large White pigs were infarcted by occlusion of the mid-left anterior descending coronary artery for 150 min. Baseline cardiac magnetic resonance (CMR) imaging with late gadolinium enhancement sequences was obtained 4 weeks post-infarction and pigs were randomized to a treatment group (intrapericardial administration of 300,000 allogeneic CDCs/kg), ( n = 10) and to a control group ( n = 9). A second CMR and high-density endocardial electroanatomical mapping were performed at 16 weeks post-infarction. After the electrophysiological study, pigs were sacrificed and epicardial optical mapping and histological studies of the heterogeneous tissue of the endocardial and epicardial scars were performed. In comparison with control conditions, intrapericardial CDCs reduced the growth of epicardial dense scar and epicardial electrical heterogeneity. The relative differences in conduction velocity and action potential duration between healthy myocardium and heterogeneous tissue were significantly smaller in the CDC-treated group than in the control group. The lower electrical heterogeneity coincides with heterogeneous tissue with less fibrosis, better cardiomyocyte viability, and a greater quantity and better polarity of connexin 43. At the endocardial level, no differences were detected between groups. Intrapericardial CDCs produce anatomical and functional changes in the epicardial arrhythmic substrate, which could have an anti-arrhythmic effect. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Carta-Bergaz, Ríos-Muñoz, Crisóstomo, Sánchez-Margallo, Ledesma-Carbayo, Bermejo-Thomas, Fernández-Avilés and Arenal-Maíz.)
Databáze:	MEDLINE
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