Phylogenetic and phylodynamic analyses of subtype-B metapneumovirus from chickens in Tunisia.

Autor:	Lachheb J; Laboratory of Epidemiology and Veterinary Microbiology, LR 11 IPT 03, Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia. Electronic address: jihene.lachheb@pasteur.tn., Bouslama Z; Laboratory of Viruses, Vectors and Hosts, LR20 IPT 02 Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia., Nsiri J; Laboratory of Epidemiology and Veterinary Microbiology, LR 11 IPT 03, Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia., Badr C; Laboratory of Epidemiology and Veterinary Microbiology, LR 11 IPT 03, Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia., Al Gallas N; Food and Water Control Laboratory, National Center for Salmonella, Shigella, and Vibriospp. Institut Pasteur de Tunis, Tunisia., Souissi N; Veterinary Research Institute of Tunis (IRVT), Tunisia., Khazri I; Veterinary Research Institute of Tunis (IRVT), Tunisia., Larbi I; Laboratory of Epidemiology and Veterinary Microbiology, LR 11 IPT 03, Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia., Kaboudi K; Department of Poultry Farming and Pathology, National Veterinary Medicine School, Sidi Thabet, Tunisia., Ghram A; Laboratory of Epidemiology and Veterinary Microbiology, LR 11 IPT 03, Institut Pasteur de Tunis, Université de Tunis El Manar (UTM), Tunisia.
Jazyk:	angličtina
Zdroj:	Poultry science [Poult Sci] 2023 Jan; Vol. 102 (1), pp. 102253. Date of Electronic Publication: 2022 Oct 14.
DOI:	10.1016/j.psj.2022.102253
Abstrakt:	Swollen Head Syndrome (SHS) is an economically important viral disease of chickens caused by avian metapneumovirus (aMPV). The virus comprises 6 different subtypes (A,B,C,D, New-1 and New-2). To date, no information was available on the presence of the virus in Tunisian poultry. The present work aims to detect the presence of (aMPV) in broiler chicken in Tunisia, then to characterise the isolates in order to determine their subtype and to estimate their geographic origin of introduction. A total of 289 samples were collected, aMPV detection was detected by real time RT-PCR and molecular characterization was warried out by Sanger sequencing on the glycoprotein (G) gene. Phylogenetic analysis was carried out using Beast 2 software. Out of the 289 samples, 21 were revealed positive to aMPV. Only 2 isolates have been confirmed by sequencing analysis ; one isolate sampled in 2015 and another in 2019. Based on the partial G gene sequence, analysis of these 2 Tunisian isolates showed that they belong to subtype B. The isolate sampled in 2015, appeared to be phylogenetically related to derived vaccine strain. However, the one sampled in 2019 appeared to be a field strain. Phylodynamic analysis provided evidence that this field strain derived from a Spanish strain and probably the virus has been introduced from Spain to North Africa back in 2016. This study is the first that highlighted the circulation of (aMPV) in Tunisia. It is possible that aMPV has been circulating in Tunisia and neighboring countries without being detected. Also, multiple strains could be present and therefore multiple introductions have happened. Through this study, we shed the light on the importance of reinforcing farms biosecurity as well as virological surveillance. (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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