Biocatalytic potential of Brassica oleracea L. var. botrytis leaves peroxidase for efficient degradation of textile dyes in aqueous medium.

Autor: Kalsoom U; Department of Chemistry, Government College Women University, Faisalabad, 38000, Pakistan., Bhatti HN; Department of Chemistry, University of Agriculture, Faisalabad, 38000, Pakistan. hnbhatti2005@yahoo.com., Aftab K; Department of Chemistry, Government College University, Faisalabad, 38000, Pakistan., Amin F; Department of Chemistry, Government College Women University, Faisalabad, 38000, Pakistan., Jesionowski T; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965, Poznan, Poland., Bilal M; Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 60965, Poznan, Poland. muhammad.bilal@put.poznan.pl.
Jazyk: angličtina
Zdroj: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2023 Mar; Vol. 46 (3), pp. 453-465. Date of Electronic Publication: 2022 Dec 01.
DOI: 10.1007/s00449-022-02820-x
Abstrakt: Dye-contaminated wastewater discharge from textile and dye manufacturing industries is reported as a world worse water polluter due to the toxic and mutagenic behavior of dyes. Peroxidase, one of the key enzymes of oxidoreductases, is widely distributed in nature and has been currently exploited in industries for various applications. Widespread applications of peroxidases are associated with their nonspecific nature towards a wide spectrum of substrates such as phenols, aromatic amines, pesticides, antibiotics, and synthetic dyes. The present study explored the potential of ammonium sulfate precipitated partially purified Brassica oleracea L. var. botrytis leaves peroxidase for degradation of reactive textile dyes Remazol Turquoise Blue 133 G and Drim Red CL4BN. Various physico-chemical parameters such as pH (2-9), temperature (20-70 ℃), enzyme activity (3-24 U/mL), concentrations of H 2 O 2 (0.4-1.4 Mm) and dye (10-100 mg/L) were optimized for enzymatic decolorization of both dyes' solution. Studies revealed that maximum degradation (95%) of Remazol Turquoise Blue 133 G with peroxidase was achieved with 25 mg/L of initial dye concentration, in the presence of 0.8 mM hydrogen peroxide with 45 min of incubation time, at pH 3, 4, and 5, and 70 °C. Maximal decolorization (97%) of Drim Red CL4BN was obtained at pH 2.0, in 10 min of incubation time at 45 ℃ using o-dianisidine hydrochloride as a redox mediator. In conclusion, the findings illustrate the prospect of Brassica oleracea peroxidase to remediate dye pollutants and dye-based industrial effluents in a green technology theme.
(© 2022. The Author(s).)
Databáze: MEDLINE
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