Characterization of the Doublesex/MAB-3 transcription factor DMD-9 in Caenorhabditis elegans.
| Autor: | Godini R; Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia., Pocock R; Development and Stem Cells Program, Monash Biomedicine Discovery Institute and Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria 3800, Australia. |
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| Jazyk: | angličtina |
| Zdroj: | G3 (Bethesda, Md.) [G3 (Bethesda)] 2023 Feb 09; Vol. 13 (2). |
| DOI: | 10.1093/g3journal/jkac305 |
| Abstrakt: | DMD-9 is a Caenorhabditis elegans Doublesex/MAB-3 Domain transcription factor (TF) of unknown function. Single-cell transcriptomics has revealed that dmd-9 is highly expressed in specific head sensory neurons, with lower levels detected in non-neuronal tissues (uterine cells and sperm). Here, we characterized endogenous dmd-9 expression and function in hermaphrodites and males to identify potential sexually dimorphic roles. In addition, we dissected the trans- and cis-regulatory mechanisms that control DMD-9 expression in neurons. Our results show that of the 22 neuronal cell fate reporters we assessed in DMD-9-expressing neurons, only the neuropeptide-encoding flp-19 gene is cell-autonomously regulated by DMD-9. Further, we did not identify defects in behaviors mediated by DMD-9 expressing neurons in dmd-9 mutants. We found that dmd-9 expression in neurons is regulated by 4 neuronal fate regulatory TFs: ETS-5, EGL-13, CHE-1, and TTX-1. In conclusion, our study characterized the DMD-9 expression pattern and regulatory logic for its control. The lack of detectable phenotypes in dmd-9 mutant animals suggests that other proteins compensate for its loss. Competing Interests: Conflicts of interest None declared. (© The Author(s) 2022. Published by Oxford University Press on behalf of the Genetics Society of America.) |
| Databáze: | MEDLINE |
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