Paired bone marrow and peripheral blood samples demonstrate lack of widespread dissemination of some CH clones.

Autor:	Osman AEG; Division of Hematology and Hematologic Malignancies, The University of Utah, Salt Lake City, UT., Mencia-Trinchant N; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Saygin C; Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, IL., Moma L; Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, IL., Kim A; Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, IL., Housman G; Department of Medicine, Section of Genetic Medicine, University of Chicago, Chicago, IL., Pozsgai M; Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, IL., Sinha E; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Chandra P; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Hassane DC; Department of Medicine, Section of Genetic Medicine, University of Chicago, Chicago, IL., Sboner A; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Sangani K; Departments of Pathology and Pediatrics, Committee on Immunology, University of Chicago, Chicago, IL., DiNardi N; Departments of Pathology and Pediatrics, Committee on Immunology, University of Chicago, Chicago, IL., Johnson C; Department of Orthopedic Surgery, University of Chicago, Chicago, IL., Wallace SS; Department of Orthopedic Surgery, University of Chicago, Chicago, IL., Jabri B; Departments of Pathology and Pediatrics, Committee on Immunology, University of Chicago, Chicago, IL., Luu H; Department of Orthopedic Surgery, University of Chicago, Chicago, IL., Guzman ML; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Desai P; Division of Hematology and Oncology, Weill Cornell Medical College, New York, NY., Godley LA; Department of Medicine, Section of Hematology/Oncology, The University of Chicago, Chicago, IL.
Jazyk:	angličtina
Zdroj:	Blood advances [Blood Adv] 2023 May 09; Vol. 7 (9), pp. 1910-1914.
DOI:	10.1182/bloodadvances.2022008521
Abstrakt:	Clonal hematopoiesis (CH) represents clonal expansion of mutated hematopoietic stem cells detectable in the peripheral blood or bone marrow through next generation sequencing. The current prevailing model posits that CH mutations detected in the peripheral blood mirror bone marrow mutations with clones widely disseminated across hematopoietic compartments. We sought to test the hypothesis that all clones are disseminated throughout hematopoietic tissues by comparing CH in hip vs peripheral blood specimens collected at the time of hip replacement surgery. Here, we show that patients with osteoarthritis have a high prevalence of CH, which involve genes encoding epigenetic modifiers and DNA damage repair pathway proteins. Importantly, we illustrate that CH, including clones with variant allele frequencies >10%, can be confined to specific bone marrow spaces and may be eliminated through surgical excision. Future work will define whether clones with somatic mutations in particular genes or clonal fractions of certain sizes are either more likely to be localized or are slower to disseminate into the peripheral blood and other bony sites. (© 2023 by The American Society of Hematology. Licensed under Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), permitting only noncommercial, nonderivative use with attribution. All other rights reserved.)
Databáze:	MEDLINE
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