Phenotyping polarization dynamics of immune cells using a lipid droplet-cell pairing microfluidic platform.

Autor:	Pinon L; École Normale Supérieure, UMR 8640, Laboratoire PASTEUR, Département de Chimie, PSL Research University, Sorbonne Université, CNRS, 75005 Paris, France.; Institut Curie, U932, Immunology and Cancer, INSERM, 75005 Paris, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, 75005 Paris, France., Ruyssen N; Arts et Métiers Institute of Technology, Université Paris 13, Sorbonne Paris Cité, IBHGC, HESAM Université, 75013 Paris, France., Pineau J; Institut Curie, U932, Immunology and Cancer, INSERM, 75005 Paris, France., Mesdjian O; École Normale Supérieure, UMR 8640, Laboratoire PASTEUR, Département de Chimie, PSL Research University, Sorbonne Université, CNRS, 75005 Paris, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, 75005 Paris, France., Cuvelier D; Institut Pierre-Gilles de Gennes pour la Microfluidique, 75005 Paris, France.; Institut Curie, UMR 144, PSL Research University, CNRS, Paris, France.; Sorbonne Université, Faculté des Sciences et Ingénierie, UFR 926 Chemistry, 75005 Paris, France., Chipont A; Institut Curie, Cytometry Platform, 75005 Paris, France., Allena R; Arts et Métiers Institute of Technology, Université Paris 13, Sorbonne Paris Cité, IBHGC, HESAM Université, 75013 Paris, France.; LJAD, UMR 7351, Université Côte d'Azur, 06100 Nice, France., Guerin CL; Institut Curie, Cytometry Platform, 75005 Paris, France.; Université Paris Cité, INSERM, Innovative Therapies in Haemostasis, 75006 Paris, France., Asnacios S; Université de Paris, CNRS, Laboratoire Matière et Systèmes Complexes, UMR 7057, 75013 Paris, France.; Sorbonne Université, Faculté des Sciences et Ingénierie, UFR 925 Physics, 75005 Paris, France., Asnacios A; Université de Paris, CNRS, Laboratoire Matière et Systèmes Complexes, UMR 7057, 75013 Paris, France., Pierobon P; Institut Curie, U932, Immunology and Cancer, INSERM, 75005 Paris, France., Fattaccioli J; École Normale Supérieure, UMR 8640, Laboratoire PASTEUR, Département de Chimie, PSL Research University, Sorbonne Université, CNRS, 75005 Paris, France.; Institut Pierre-Gilles de Gennes pour la Microfluidique, 75005 Paris, France.
Jazyk:	angličtina
Zdroj:	Cell reports methods [Cell Rep Methods] 2022 Nov 09; Vol. 2 (11), pp. 100335. Date of Electronic Publication: 2022 Nov 09 (Print Publication: 2022).
DOI:	10.1016/j.crmeth.2022.100335
Abstrakt:	The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formation of the synapse, their impact on polarization has not yet been studied. Using functional lipid droplets as tunable artificial presenting cells combined with a microfluidic pairing device, we simultaneously observe synchronized synapses and dynamically quantify polarization patterns of individual B cells. By assessing how ligand concentration, surface fluidity, and substrate rigidity impact lysosome polarization, we show that its onset and kinetics depend on the local antigen concentration at the synapse and on substrate rigidity. Our experimental system enables a fine phenotyping of monoclonal cell populations based on their synaptic readout. Competing Interests: The authors declare no competing interests. (© 2022 The Authors.)
Databáze:	MEDLINE
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