Immunologic and pathologic characterization of a novel swine biomedical research model for eosinophilic esophagitis.

Autor:	Cortes LM; Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States., Brodsky D; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States.; Department of Biological Sciences, North Carolina State University, Raleigh, NC, United States., Chen C; USDA, ARS, Diet, Genomics and Immunology Laboratory, Beltsville, MD, United States., Pridgen T; Department of Clinical Sciences, North Carolina State University, Raleigh, NC, United States., Odle J; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States.; Laboratory of Developmental Nutrition, Department of Animal Science, North Carolina State University, Raleigh, NC, United States., Snider DB; Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, United States., Cruse G; Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, United States., Putikova A; Division of Allergy, Asthma, and Clinical Immunology, Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, United States., Masuda MY; Division of Allergy, Asthma, and Clinical Immunology, Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, United States.; Department of Immunology, Mayo Clinic, Rochester, MN, United States.; Department of Immunology, Mayo Clinic Arizona, Scottsdale, AZ, United States., Doyle AD; Division of Allergy, Asthma, and Clinical Immunology, Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, United States., Wright BL; Division of Allergy, Asthma, and Clinical Immunology, Department of Medicine, Mayo Clinic Arizona, Scottsdale, AZ, United States.; Section of Allergy and Immunology, Division of Pulmonology, Phoenix Children's Hospital, Phoenix, AZ, United States., Dawson HD; USDA, ARS, Diet, Genomics and Immunology Laboratory, Beltsville, MD, United States., Blikslager A; Department of Clinical Sciences, North Carolina State University, Raleigh, NC, United States., Dellon ES; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States.; Division of Gastroenterology and Hepatology, Department of Medicine, Center for Esophageal Diseases and Swallowing, University of North Carolina School of Medicine, Chapel Hill, NC, United States., Laster SM; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States.; Department of Biological Sciences, North Carolina State University, Raleigh, NC, United States., Käser T; Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States.; Center for Food Allergy Modeling in Pigs (CFAMP), Comparative Medicine Institute, North Carolina State University, Raleigh, NC, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in allergy [Front Allergy] 2022 Nov 14; Vol. 3, pp. 1029184. Date of Electronic Publication: 2022 Nov 14 (Print Publication: 2022).
DOI:	10.3389/falgy.2022.1029184
Abstrakt:	Eosinophilic esophagitis (EoE) is a chronic allergy-mediated condition with an increasing incidence in both children and adults. Despite EoE's strong impact on human health and welfare, there is a large unmet need for treatments with only one recently FDA-approved medication for EoE. The goal of this study was to establish swine as a relevant large animal model for translational biomedical research in EoE with the potential to facilitate development of therapeutics. We recently showed that after intraperitoneal sensitization and oral challenge with the food allergen hen egg white protein (HEWP), swine develop esophageal eosinophilia-a hallmark of human EoE. Herein, we used a similar sensitization and challenge treatment and evaluated immunological and pathological markers associated with human EoE. Our data demonstrate that the incorporated sensitization and challenge treatment induces (i) a systemic T-helper 2 and IgE response, (ii) a local expression of eotaxin-1 and other allergy-related immune markers, (iii) esophageal eosinophilia (>15 eosinophils/0.24 mm 2 ), and (iv) esophageal endoscopic findings including linear furrows and white exudates. Thereby, we demonstrate that our sensitization and oral challenge protocol not only induces the underlying immune markers but also the micro- and macro-pathological hallmarks of human EoE. This swine model for EoE represents a novel relevant large animal model that can drive translational biomedical research to develop urgently needed treatment strategies for EoE. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2022 Cortes, Brodsky, Chen, Pridgen, Odle, Snider, Cruse, Putikova, Masuda, Doyle, Wright, Dawson, Blikslager, Dellon, Laster and Käser.)
Databáze:	MEDLINE
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