SARS-CoV-2 evolves increased infection elicited cell death and fusion in an immunosuppressed individual.

Autor:	Lustig G; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa., Ganga Y; Africa Health Research Institute, Durban, South Africa., Rodel H; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa.; Division of Infection and Immunity, University College London, London, UK., Tegally H; KwaZulu-Natal Research Innovation and Sequencing Platform, Durban, South Africa., Jackson L; Africa Health Research Institute, Durban, South Africa., Cele S; Africa Health Research Institute, Durban, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Khan K; Africa Health Research Institute, Durban, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Jule Z; Africa Health Research Institute, Durban, South Africa., Reedoy K; Africa Health Research Institute, Durban, South Africa., Karim F; Africa Health Research Institute, Durban, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Bernstein M; Africa Health Research Institute, Durban, South Africa., Moosa MS; Department of Infectious Diseases, Nelson R. Mandela School of Clinical Medicine, University of KwaZulu-Natal, Durban, South Africa., Archary D; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa., de Oliveira T; KwaZulu-Natal Research Innovation and Sequencing Platform, Durban, South Africa.; Centre for Epidemic Response and Innovation (CERI), School of Data Science and Computational Thinking, Stellenbosch University, Stellenbosch, South Africa.; Department of Global Health, University of Washington, Seattle, USA., Lessells R; KwaZulu-Natal Research Innovation and Sequencing Platform, Durban, South Africa., Abdool Karim SS; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa.; Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, NY, United States., Sigal A; Centre for the AIDS Programme of Research in South Africa, Durban, South Africa.; Africa Health Research Institute, Durban, South Africa.; School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2022 Nov 30. Date of Electronic Publication: 2022 Nov 30.
DOI:	10.1101/2022.11.23.22282673
Abstrakt:	The milder clinical manifestations of Omicron infection relative to pre-Omicron SARS CoV-2 raises the possibility that extensive evolution results in reduced pathogenicity. To test this hypothesis, we quantified induction of cell fusion and cell death in SARS CoV-2 evolved from ancestral virus during long-term infection. Both cell fusion and death were reduced in Omicron BA.1 infection relative to ancestral virus. Evolved virus was isolated at different times during a 6-month infection in an immunosuppressed individual with advanced HIV disease. The virus isolated 16 days post-reported symptom onset induced fusogenicity and cell death at levels similar to BA.1. However, fusogenicity was increased in virus isolated at 6 months post-symptoms to levels intermediate between BA.1 and ancestral SARS-CoV-2. Similarly, infected cell death showed a graded increase from earlier to later isolates. These results may indicate that, at least by the cellular measures used here, evolution in long-term infection does not necessarily attenuate the virus. Competing Interests: Competing interest statement AS received an honorarium for a talk given to Pfizer employees.
Databáze:	MEDLINE
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