Evaluating the clinical significance of FLT3 mutation status in Syrian newly diagnosed acute myeloid leukemia patients with normal karyotype.

Autor:	Moualla Y; Department of Laboratory Diagnosis, Faculty of Pharmacy, Tishreen University, Ministry of Higher Education, Lattakia, Syria., Moassass F; Department of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria., Al-Halabi B; Department of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria., Al-Achkar W; Department of Molecular Biology and Biotechnology, Human Genetics Division, Atomic Energy Commission, Damascus, Syria., Georgeos M; Department of Hematology and Oncology, Faculty of Medicine, Tishreen University, Ministry of Higher Education, Lattakia, Syria., Yazigi H; Department of Laboratory Diagnosis, Faculty of Medicine, Tishreen University, Ministry of Higher Education, Lattakia, Syria., Khamis A; Department of Laboratory Diagnosis, Faculty of Pharmacy, Tishreen University, Ministry of Higher Education, Lattakia, Syria.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2022 Nov 25; Vol. 8 (11), pp. e11858. Date of Electronic Publication: 2022 Nov 25 (Print Publication: 2022).
DOI:	10.1016/j.heliyon.2022.e11858
Abstrakt:	The FMS-like tyrosine kinase-3 internal tandem duplication ( FLT3 -ITD) is one of the most prevalent mutations, affecting between 20 and 30 percent of cases in patients with acute myeloid leukemia (AML). The Patients with a FLT3 -ITD mutation have a poor prognosis. In the present study, we investigated the FLT3 (ITD-TKD) mutations in 100 newly adult Syrian patients with AML-Normal karyotype (NK). Our results revealed that prevalence of FLT3 -ITD mutation was 24%. Interestingly, 20 patients had a typical duplication mutation and four patients had different mutations. From those four mentioned patients, two of them carried a 39 base pair (bp) duplication in different location: (c.1838_1877dup39, p.591-603dup) and (c.1836_1874 dup 39, p.591-603dup), the third patient, showed FLT3 -ITD duplication and a newly insertion together, this insertion was not demonstrated before: (c.1842_1865dup24, c.1865_1866insGAA). Finally, the fourth patient exhibited a duplication of 21bp (c.1855_1875dup21, p.597-603dup). In addition, statistically significant differences were observed for the relation between the presence of FLT3 -ITD mutation and lactate dehydrogenase (LDH) level, overall survival (OS), relapse, and event free survival (EFS). We demonstrated that our patients with FLT3 -ITD mutation had a poor prognosis. Also, the frequency of FLT3 -TKD mutation was low 2% and no compound between the two mutations was found, as individuals showed to carry the two mutations were not detected. These findings are likely useful for a better understanding of molecular leukemogenetic steps in AML-NK patients and may be beneficial for clinical relevance for risk grouping, study design and choice of therapy in Syrian population. Competing Interests: The authors declare no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
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