Antimicrobial and Cytotoxic Potential of Helminthosporin from Rumex abyssiniscus Jacq. Discovered as a Novel Source of Syringic Acid and Bis(2-ethyloctyl) Phthalate.

Autor: Ntemafack A; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, India. ntemafackbc4@yahoo.fr.; Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India. ntemafackbc4@yahoo.fr.; Department of Biochemistry and Molecular Biology, Indiana University-Purdue University, Indianapolis, IN, USA. ntemafackbc4@yahoo.fr., Qayum A; Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India., Dhiman SK; Instrumentation Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India., Guefack MF; Department of Biochemistry, University of Dschang, Dschang, Cameroon., Thapa S; Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India., Wamba BEN; Department of Biochemistry, University of Dschang, Dschang, Cameroon., Kuete V; Department of Biochemistry, University of Dschang, Dschang, Cameroon., Singh SK; Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India., Bharate SB; Medicinal Chemistry Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India., Hassan QP; Molecular Biology and Plant Biotechnology Division, CSIR- Indian Institute of Integrative Medicine, Sanat Nagar, Srinagar, India. qphassan@iiim.res.in., Gandhi SG; Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India. sumit@iiim.res.in.
Jazyk: angličtina
Zdroj: Current microbiology [Curr Microbiol] 2022 Nov 29; Vol. 80 (1), pp. 7. Date of Electronic Publication: 2022 Nov 29.
DOI: 10.1007/s00284-022-03101-2
Abstrakt: Rumex abyssinicus Jacq. is a perennial medicinal herb widely used in traditional medicine to treat many diseases. Phytochemicals of the plant were isolated using column chromatography and thin layer chromatography techniques. Extract, fractions and pure compounds were screened for antimicrobial activity against sensitive and multi-drug resistant microbes and their cytotoxicity was performed on different cancer cell lines. The mechanism of action of purified helminthosporin as well as the potent fraction containing a mixture of two compounds was assessed. Fraction R7C3 was the most potent antibacterial with the lowest MIC value of 0.12 µg/mL. Helminthosporin was the most potent compound with the lowest MIC value of 1.95 µg/mL. The compound was more potent than the antibiotic chloramphenicol against multi-drug resistant (MDR) bacteria with MIC equal to 16 µg/mL. The fraction and helminthosporin were shown to destroy the cell wall of the yeast and bacteria, and DNA fragmentation effect on the genome of Candida albicans and Bacillus cereus. Helminthosporin was the most cytotoxic compound with IC 50  ˂ 10 µM. Fraction R7C3 showed the most potent cytotoxic effects on all cancer cell lines, with IC 50 ranging from ˂1 to 4.35 ng/mL. Our study is the first report on the mechanism of action of helminthosporin, a potent candidate in the development of new drugs against multi-resistant bacteria and cancer cells. In addition, this study uncovered Rumex abyssinicus as a new source of syringic acid and bis(2-ethyloctyl) phthalate.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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