Reversible adhesion by type IV pili leads to formation of permanent localized clusters.

Autor:	Pepe MV; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Dea C; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Genskowsky C; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Capasso D; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Roset MS; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Jäger AV; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina., Peruani F; Laboratoire de Physique Théorique et Modélisation, UMR 8089, CY Cergy Paris Université, 95302 Cergy-Pontoise, France., Kierbel A; Instituto de Investigaciones Biotecnológicas, Universidad Nacional de San Martín (UNSAM), CONICET, Buenos Aires B1650HMP, Argentina.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2022 Nov 09; Vol. 25 (12), pp. 105532. Date of Electronic Publication: 2022 Nov 09 (Print Publication: 2022).
DOI:	10.1016/j.isci.2022.105532
Abstrakt:	The formation of long-lived, multicellular clusters is a fundamental step in the physiopathology of many disease-causing bacteria. Experiments on abiotic surfaces suggest that bacterial colonization, including initial cluster formation, requires (1) irreversible adhesion, (2) cell proliferation, and (3) a phenotypic transition. However, here we show that on infection of a polarized MDCK epithelium, Pseudomonas aeruginosa (PA) forms long-lived - i.e., permanent  - bacterial clusters without requiring irreversible adhesion, cell proliferation, or a phenotypic transition. By combining experiments and a mathematical model, we reveal that the cluster formation process is mediated by type IV pili (T4P). Furthermore, we unveil how T4P quantitatively operate during adhesion, finding that it is a stochastic process that involves an activation time, requires the retraction of pili, and results in reversible attachment. We explain how such reversible attachment process leads to the formation of permanent bacterial clusters and quantify the cluster growth dynamics. Competing Interests: The authors declare no competing interests. (© 2022 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu