Genomic analysis and in vivo efficacy of Pediococcus acidilactici as a potential probiotic to prevent hyperglycemia, hypercholesterolemia and gastrointestinal infections.

Autor: Al-Emran HM; Department Biomedical Engineering, Faculty of Engineering, Jashore University of Science and Technology, Jashore, Bangladesh., Moon JF; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Miah ML; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Meghla NS; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Reuben RC; German Centre for Integrative Biodiversity Research (iDiv), Halle-Jena-Leipzig, Germany., Uddin MJ; Department of Pharmacy, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Ibnat H; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Sarkar SL; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Roy PC; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Rahman MS; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Alam ASMRU; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Islam OK; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh., Jahid IK; Department of Microbiology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, Bangladesh. ikjahid_mb@just.edu.bd.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Nov 28; Vol. 12 (1), pp. 20429. Date of Electronic Publication: 2022 Nov 28.
DOI: 10.1038/s41598-022-24791-5
Abstrakt: Lactic acid bacteria are the well acknowledged probiotics that can cure a variety of diseases. In this study, we observed the in vivo potentials of Pediococcus to treat hyperglycemia, hypercholesterolemia and gastrointestinal infections. A total of 77 Lactobacillus were isolated from the milk of 10 cows and 10 goats, four of those strains inhibited both carbohydrates-hydrolyzing enzymes, α-glucosidase, and α-amylase. They all showed antagonistic effects on pathogenic E. coli and S. Typhimurium which were confirmed by performing pathogen challenge test and visualizing on Electron microscopy. 16S rRNA gene sequence identified that all four strains belong to Pediococcus genus which were further distinguished as Pediococcus acidilactici by pheS gene sequence. Whole genome sequence analysis revealed their non-pathogenic properties for human and the presence of probiotic genes responsible for stress resistance, immunomodulation, adhesion, metal and drug resistance. In vivo trial with diabetes-induced mice ascertained that all Pediococcus acidilactici had significant potentials to reduce elevated glucose and low-density lipoprotein level in blood. Interestingly, two out of four strains were significantly more effective (p < 0.0001 each) than metformin in reducing the blood glucose level. This in vivo study demonstrated that Pediococcus acidilactici might be a promising probiotic to prevent hyperglycemia, hypercholesterolemia and gastrointestinal infections.
(© 2022. The Author(s).)
Databáze: MEDLINE
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