Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers.

Autor: Soriano Pérez ML; Grupo de Sanidad Animal, INTA Marcos Juárez, Córdoba, Argentina., Funes JA; Grupo de Sanidad Animal, INTA Marcos Juárez, Córdoba, Argentina., Flores Bracamonte C; INCIVET CONICET-UNRC, Rio Cuarto, Argentina., Ibarra LE; Instituto de Biotecnología Ambiental y Salud (INBIAS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina., Forrellad MA; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), UEDD INTA-CONICET, CICVyA, Hurlingham, Buenos Aires, Argentina., Taboga O; Instituto de Agrobiotecnología y Biología Molecular (IABIMO), UEDD INTA-CONICET, CICVyA, Hurlingham, Buenos Aires, Argentina., Cariddi LN; Instituto de Biotecnología Ambiental y Salud (INBIAS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina., Salinas FJ; CMC, ICIVET-Litoral, UNL-CONICET, Esperanza, Argentina., Ortega HH; CMC, ICIVET-Litoral, UNL-CONICET, Esperanza, Argentina., Alustiza F; Grupo de Sanidad Animal, INTA Marcos Juárez, Córdoba, Argentina., Molina M; IITEMA CONICET-UNRC, Río Cuarto, Córdoba, Argentina. Electronic address: mamolina7@gmail.com.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2023 Jan 05; Vol. 630, pp. 122435. Date of Electronic Publication: 2022 Nov 26.
DOI: 10.1016/j.ijpharm.2022.122435
Abstrakt: "Smart" nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N-isopropylacrylamide) (pNIPAM) and theirin vitro, ex vivoand in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluatepNIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor ofActinobacillus pleuropneumoniae(App)cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution ofpNIPAM nanogels administered intranasally was performedinvivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studiedin vivoby measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated intopNIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated thatpNIPAM nanogels can be used as an efficient platform for vaccine nanocarriers.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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