The Nox2-ROS-Nlrp3 Inflammasome Signaling Stimulates in the Hematopoietic Stem/Progenitor Cells Lipogenesis to Facilitate Membrane Lipid Raft Formation.

Autor: Abdelbaset-Ismail A; Laboratory of Regenerative Medicine, Medical University of Warsaw, Warsaw, Poland.; Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt., Ciechanowicz AK; Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA.; Department of Regenerative Medicine, Center for Preclinical Studies and Technology, Medical University of Warsaw, Warsaw, Poland., Bujko K; Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA.; Department of Regenerative Medicine, Center for Preclinical Studies and Technology, Medical University of Warsaw, Warsaw, Poland., Ratajczak J; Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA., Kucia M; Laboratory of Regenerative Medicine, Medical University of Warsaw, Warsaw, Poland.; Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA., Ratajczak MZ; Laboratory of Regenerative Medicine, Medical University of Warsaw, Warsaw, Poland. mzrata01@louisville.edu.; Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA. mzrata01@louisville.edu.; Department of Regenerative Medicine, Center for Preclinical Studies and Technology, Medical University of Warsaw, Warsaw, Poland. mzrata01@louisville.edu.
Jazyk: angličtina
Zdroj: Stem cell reviews and reports [Stem Cell Rev Rep] 2023 Jan; Vol. 19 (1), pp. 92-103. Date of Electronic Publication: 2022 Nov 28.
DOI: 10.1007/s12015-022-10481-2
Abstrakt: Proliferation, metabolism, and migration of hematopoietic stem/progenitor cells (HSPCs) are coordinated by receptors expressed on outer cell membranes that are integrated into microdomains, known as membrane lipid rafts (MLRs). These structures float freely in the cell membrane bilayer and are enriched in cholesterol and sphingolipids for their functional integrity. Receptors, if expressed in MLRs, have prolonged occupancy on the cell surface and enhanced signaling power. Based on this, we have become interested in the regulation of synthesis of MLRs components in HSPCs. To address this, we tested the effect of selected factors that promote proliferation or migration and their potential involvement in the synthesis of MLRs components in HSPCs. Based on our previous research showing that HSPCs from Nox2-KO and Nlrp3-KO mice display a profound defect in MLRs formation, we focused on the role of Nox2-ROS-Nlrp3 inflammasome in regulating lipogenesis in HSPCs. We found that while at steady state conditions, Nox2-derived ROS is required for a proper expression of enzymes regulating lipogenesis, during inflammation, this effect is augmented by Nlrp3 inflammasome. Thus, our data sheds new light on the regulation of lipogenesis in HSPCs and the involvement of the Nox2-ROS-Nlrp3 inflammasome axis that differently regulates lipogenesis at steady state conditions and in response to inflammation, modulating MLRs-mediated responsiveness of these cells to external stimuli.
(© 2022. The Author(s).)
Databáze: MEDLINE
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