Exosomal MicroRNAs: Comprehensive Methods from Exosome Isolation to miRNA Extraction and Purity Analysis.

Autor:	D'Agostino E; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy., Muro A; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy.; Università degli studi di Napoli 'Federico II', Naples, Italy., Sgueglia G; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy., Massaro C; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy., Dell'Aversana C; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy. carmela.dellaversana@cnr.it.; Institute Experimental Endocrinology and Oncology 'Gaetano Salvatore' (IEOS)-National Research Council (CNR), Naples, Italy. carmela.dellaversana@cnr.it., Altucci L; Department of Precision Medicine, Università degli studi della Campania 'Luigi Vanvitelli', Naples, Italy. lucia.altucci@unicampania.it.; BIOGEM, Ariano Irpino, Italy. lucia.altucci@unicampania.it.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2595, pp. 75-92.
DOI:	10.1007/978-1-0716-2823-2_5
Abstrakt:	Exosomes are extracellular vesicles secreted by cells with a key role in a wide range of biological processes including cancer. These vesicles are involved in intercellular communication and deliver diverse cargo molecules, including miRNAs (exo-miRNAs), to recipient cells affecting their physiology. Exo-miRNAs have a role in promoting tumor, progression, metastatization, and remodeling of tumor microenvironment, therefore making them interesting biomarkers to study.Here we provide a detailed technical protocol for exosome isolation (which can be applied to cell culture as well as physiological fluids), validation of their vesicular identity, miRNA extraction, and quantitative and qualitative analysis to evaluate the sample purity and concentration. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu