Production of C20 9S- and C22 11S-hydroxy fatty acids by cells expressing Shewanella hanedai arachidonate 9S-lipoxygenase.
Autor: | Kim MJ; Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea., Lee J; Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea., Kim SE; Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea., Shin KC; Department of Integrative Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea., Oh DK; Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea. deokkun@konkuk.ac.kr.; Department of Integrative Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea. deokkun@konkuk.ac.kr. |
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Jazyk: | angličtina |
Zdroj: | Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Jan; Vol. 107 (1), pp. 247-260. Date of Electronic Publication: 2022 Nov 28. |
DOI: | 10.1007/s00253-022-12285-3 |
Abstrakt: | The putative lipoxygenase (LOX) from the proteobacterium Shewanella hanedai was determined to be an 82 kDa monomeric enzyme by SDS-PAGE and gel filtration chromatography analysis. LOX was identified as a single-dioxygenating arachidonate (ARA) 9S-LOX by analyzing ARA-derived bioconversion products using high-performance liquid chromatography with reverse-, normal-, and chiral-phase columns and evaluating kinetic parameters for C20- and C22-polyunsaturated fatty acids (PUFAs). The catalytic efficiency (k (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
Databáze: | MEDLINE |
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