Establishment and optimization of a new model organism to study early land plant evolution: Germination, cultivation and oospore variation of Chara braunii Gmelin, 1826.

Autor:	Holzhausen A; Department of Biology, Plant Cell Biology, University of Marburg, Marburg, Germany.; Institute for Biosciences, Physiology of Plant Metabolism, University of Rostock, Rostock, Germany., Stingl N; Department of Biology, Plant Cell Biology, University of Marburg, Marburg, Germany., Rieth S; Department of Biology, Plant Cell Biology, University of Marburg, Marburg, Germany., Kühn C; Department of Biology, Plant Cell Biology, University of Marburg, Marburg, Germany.; Institute for Biosciences, Ecology, University of Rostock, Rostock, Germany., Schubert H; Institute for Biosciences, Physiology of Plant Metabolism, University of Rostock, Rostock, Germany., Rensing SA; Department of Biology, Plant Cell Biology, University of Marburg, Marburg, Germany.; BIOSS Centre for Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2022 Nov 10; Vol. 13, pp. 987741. Date of Electronic Publication: 2022 Nov 10 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.987741
Abstrakt:	For studying land plant evolution, the establishment and optimization of model organisms representing streptophytic algae, sister to land plants, is essential. Long-term cultivation experiments with Chara braunii S276 were performed over 8 years, since 4 years (Nov. 2018) under constant conditions. Additionally, short-term experiments for optimization of culture conditions were performed with three strains of C. braunii (S276, NIES-1604 and Lausiger Teiche, LaT-2708). Germination success after application of sterilization agents, addition of gibberellic acid and under different incubation conditions with respect to pre-treatment, irradiance regime and substrate was investigated in order to develop protocols for generative cultivation of at least unialgal cultures. The resulting cultivation protocols for C. braunii S276, allowing maintenance of vegetative as well as generative cultures are presented in detail, including protocols for germination induction and growth of sterilized and unsterilized oospores. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Holzhausen, Stingl, Rieth, Kühn, Schubert and Rensing.)
Databáze:	MEDLINE
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