Pyridine and isoxazole substituted 3-formylindole-based chitosan Schiff base polymer: Antimicrobial, antioxidant and in vitro cytotoxicity studies on THP-1 cells.

Autor: Ali MA; Department of Chemistry, The New College (Autonomous), Chennai 600014, Tamil Nadu, India; Department of Chemistry, Presidency College (Autonomous), Chennai 600005, Tamil Nadu, India., Aswathy KA; Division of Molecular Biology and Immuno Biology, IIISM, SRM IST, Kattankulathur, 603203, Tamil Nadu, India., Munuswamy-Ramanujam G; Division of Molecular Biology and Immuno Biology, IIISM, SRM IST, Kattankulathur, 603203, Tamil Nadu, India; Department of Chemistry, Faculty of Science & Humanities, SRM IST, Kattankulathur, 603203, Tamil Nadu, India., Jaisankar V; Department of Chemistry, Presidency College (Autonomous), Chennai 600005, Tamil Nadu, India. Electronic address: vjaisankar@gmail.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Jan 15; Vol. 225, pp. 1575-1587. Date of Electronic Publication: 2022 Nov 25.
DOI: 10.1016/j.ijbiomac.2022.11.214
Abstrakt: This paper presents the synthesis of two new chitosan Schiff base (CSB) polymers, namely, 2PCT and 4MCT based on pyridin-2-ylmethyl-1H-indole-3-carbaldehyde and 1-(4-methyl-3,5-dimethylisoxazole)-1H-indole-3-carbaldehyde with chitosan (CT). The structural features of CSB polymers were confirmed by Fourier-transform infrared (FTIR) and proton nuclear magnetic resonance ( 1 H NMR) spectroscopy and their antimicrobial activity was evaluated against Staphylococcus aureus, Escherichia coli and Candida albicans. The antioxidant studies found that both 2PCT and 4MCT presented significant free radical scavenging activity with IC 50 at 169.01 and 372.84 μg/mL, respectively. The cell viability results obtained from in vitro cytotoxicity studies performed using human monocyte leukemia (THP-1) cells were found to be 75.6 ± 0.25 % and 79.1 ± 1.5 % for 2PCT and 4MCT, respectively, at a concentration of 10 mg/mL. Flow cytometry analysis demonstrated the reducing ability of CSB polymers on intracellular reactive oxygen species (ROS) in THP-1 cells. The overall results of antioxidant activity, in vitro biocompatibility and ability to reduce the intracellular ROS production emphasized that the CSB polymers prepared could serve as a potential biomaterial in biomedical applications, such as wound treatment process.
Competing Interests: Declaration of competing interest None.
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Databáze: MEDLINE
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