Production of cytoplasmic type citrus leprosis virus-like particles by plant molecular farming.

Autor: Ortega-Rivera OA; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA., Beiss V; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA., Osota EO; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA., Chan SK; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA., Karan S; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA., Steinmetz NF; Department of NanoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Center for Nano-ImmunoEngineering, University of California-San Diego, La Jolla, CA, 92039, USA; Institute for Materials Discovery and Design, University of California-San Diego, La Jolla, CA, 92039, USA; Department of Bioengineering, University of California-San Diego, La Jolla, CA, 92039, USA; Department of Radiology, University of California-San Diego, La Jolla, CA, 92039, USA; Moores Cancer Center, University of California-San Diego, La Jolla, CA, 92039, USA. Electronic address: nsteinmetz@ucsd.edu.
Jazyk: angličtina
Zdroj: Virology [Virology] 2023 Jan; Vol. 578, pp. 7-12. Date of Electronic Publication: 2022 Nov 15.
DOI: 10.1016/j.virol.2022.11.004
Abstrakt: Many plant virus-like particles (VLPs) utilized in nanotechnology are 30-nm icosahedrons. To expand the VLP platforms, we produced VLPs of Cytoplasmic type citrus leprosis virus (CiLV-C) in Nicotiana benthamiana. We were interested in CiLV-C because of its unique bacilliform shape (60-70 nm × 110-120 nm). The CiLV-C capsid protein (p29) gene was transferred to the pTRBO expression vector transiently expressed in leaves. Stable VLPs were formed, as confirmed by agarose gel electrophoresis, transmission electron microscopy and size exclusion chromatography. Interestingly, the morphology of the VLPs (15.8 ± 1.3 nm icosahedral particles) differed from that of the native bacilliform particles indicating that the assembly of native virions is influenced by other viral proteins and/or the packaged viral genome. The smaller CiLV-C VLPs will also be useful for structure-function studies to compare with the 30-nm icosahedrons of other VLPs.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Dr. Steinmetz is a co-founder of, has equity in, and has a financial interest with Mosaic ImmunoEngineering Inc. Dr. Steinmetz serves as Director, Board Member, and Acting Chief Scientific Officer, and paid consultant to Mosaic. The other authors declare no potential conflict of interest.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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