Basophil activation test has high reproducibility and is feasible in the clinical setting.

Autor: Jaumdally H; Department of Women and Children's Health (Pediatric Allergy), School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.; Children's Allergy Service, Evelina London Children's Hospital, Guy's and St Thomas' Hospital, London, UK., Kwok M; Department of Women and Children's Health (Pediatric Allergy), School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.; Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK., Jama Z; Department of Women and Children's Health (Pediatric Allergy), School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.; Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK., Hesse-Lamptey R; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK., McKendry R; Department of Women and Children's Health (Pediatric Allergy), School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.; Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK., Galvez O; Viapath, Special Haematology Laboratory, London, UK., Daniel Y; Viapath, Special Haematology Laboratory, London, UK., Santos AF; Department of Women and Children's Health (Pediatric Allergy), School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London, London, UK.; Peter Gorer Department of Immunobiology, School of Immunology and Microbial Sciences, King's College London, London, UK.; Children's Allergy Service, Evelina London Children's Hospital, Guy's and St Thomas' Hospital, London, UK.; Asthma UK Centre in Allergic Mechanisms of Asthma, London, UK.
Jazyk: angličtina
Zdroj: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology [Pediatr Allergy Immunol] 2022 Oct; Vol. 33 (11), pp. e13870.
DOI: 10.1111/pai.13870
Abstrakt: Background: The basophil activation test (BAT) has high accuracy to diagnose peanut allergy and can reduce the need for oral food challenges (OFC); however, so far it has not been incorporated in clinical practice.
Methods: We assessed the reproducibility of BAT within the same laboratory and between two different laboratories and the feasibility of using BAT in the clinical setting.
Results: One hundred and two children being assessed for peanut allergy were tested on BAT (72 allergic, 30 sensitized tolerant). There was little internal variation (coefficient of variation <15%) in the BAT and a very strong correlation (R s  > .95) between BAT performed across laboratories. The 2 BAT methods were strongly correlated but not interchangeable. In the cases of discrepancy, our in house BAT method was 100% accurate. BAT was feasible and well-accepted by clinicians: no patient with positive BAT was referred for OFC, leading to reduction in the number of OFC required. Twenty one percent of patients who underwent OFC reacted to peanut. A negative BAT also encouraged the performance of OFC in sensitized children who would otherwise be considered allergic, 50% of whom did not react and incorporated peanut in the diet.
Conclusions: The BAT is a robust test that can reliably be transferred between laboratories; however, different BAT methods are not interchangeable. BAT was well integrated in the clinical decision-making process in a specialized center.
(© 2022 The Authors. Pediatric Allergy and Immunology published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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