Peripheral T-Cells, B-Cells, and Monocytes from Multiple Sclerosis Patients Supplemented with High-Dose Vitamin D Show Distinct Changes in Gene Expression Profiles.

Autor:	Kim D; Neurology and Neurological Sciences Department, Stanford University School of Medicine, Stanford, CA 94305, USA., Witt EE; Harvard Medical School, Boston, MA 02115, USA., Schubert S; Department of Environmental Health and Safety, Stanford University School of Medicine, Stanford, CA 94305, USA., Sotirchos E; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Bhargava P; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Mowry EM; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Sachs K; Next Generation Analytics, Palo Alto, CA 94301, USA., Bilen B; Data Science and Engineering Consultant, Mountain View, CA 94041, USA., Steinman L; Neurology and Neurological Sciences Department, Stanford University School of Medicine, Stanford, CA 94305, USA., Awani A; Neurology and Neurological Sciences Department, Stanford University School of Medicine, Stanford, CA 94305, USA., He Z; Neurology and Neurological Sciences Department, Stanford University School of Medicine, Stanford, CA 94305, USA., Calabresi PA; Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA., Van Haren K; Neurology and Neurological Sciences Department, Stanford University School of Medicine, Stanford, CA 94305, USA.
Jazyk:	angličtina
Zdroj:	Nutrients [Nutrients] 2022 Nov 09; Vol. 14 (22). Date of Electronic Publication: 2022 Nov 09.
DOI:	10.3390/nu14224737
Abstrakt:	Vitamin D is a steroid hormone that has been widely studied as a potential therapy for multiple sclerosis and other inflammatory disorders. Pre-clinical studies have implicated vitamin D in the transcription of thousands of genes, but its influence may vary by cell type. A handful of clinical studies have failed to identify an in vivo gene expression signature when using bulk analysis of all peripheral immune cells. We hypothesized that vitamin D's gene signature would vary by immune cell type, requiring the analysis of distinct cell types. Multiple sclerosis patients ( n = 18) were given high-dose vitamin D (10,400 IU/day) for six months as part of a prospective clinical trial (NCT01024777). We collected peripheral blood mononuclear cells from participants at baseline and again after six months of treatment. We used flow cytometry to isolate three immune cell types (CD4 + T-cells, CD19 + B-cells, CD14 + monocytes) for RNA microarray analysis and compared the expression profiles between baseline and six months. We identified distinct sets of differentially expressed genes and enriched pathways between baseline and six months for each cell type. Vitamin D's in vivo gene expression profile in the immune system likely differs by cell type. Future clinical studies should consider techniques that allow for a similar cell-type resolution.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu