| Autor: |
Vizitiu DE; The National Institute for Research & Development for Biotechnology in Horticulture Stefanesti, 110134 Stefanesti, Romania., Sardarescu DI; The National Institute for Research & Development for Biotechnology in Horticulture Stefanesti, 110134 Stefanesti, Romania.; Faculty of Chemical Engineering and Biotechnologies, University 'Politehnica' of Bucharest, Bucharest, 313 Splaiul Independentei Str., 060042 Bucharest, Romania., Fierascu I; The National Institute for Research & Development in Chemistry and Petrochemistry, ICECHIM, 202 Spl. Independentei, 060021 Bucharest, Romania.; Faculty of Horticulture, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 011464 Bucharest, Romania., Fierascu RC; Faculty of Chemical Engineering and Biotechnologies, University 'Politehnica' of Bucharest, Bucharest, 313 Splaiul Independentei Str., 060042 Bucharest, Romania.; The National Institute for Research & Development in Chemistry and Petrochemistry, ICECHIM, 202 Spl. Independentei, 060021 Bucharest, Romania., Soare LC; Natural Science Department, Faculty of Sciences, Physical Education and Informatics, University of Pitesti, 110040 Pitesti, Romania., Ungureanu C; Faculty of Chemical Engineering and Biotechnologies, University 'Politehnica' of Bucharest, Bucharest, 313 Splaiul Independentei Str., 060042 Bucharest, Romania., Buciumeanu EC; The National Institute for Research & Development for Biotechnology in Horticulture Stefanesti, 110134 Stefanesti, Romania., Guta IC; The National Institute for Research & Development for Biotechnology in Horticulture Stefanesti, 110134 Stefanesti, Romania., Pandelea LM; The National Institute for Research & Development for Biotechnology in Horticulture Stefanesti, 110134 Stefanesti, Romania. |
| Abstrakt: |
Starting from the well-known antimicrobial properties of silver nanoparticles, the goal of this study is to evaluate the influence of two "green" recipes, namely an alcoholic extract of Dryopteris filix-mas (L.) Schott and a dispersion of silver nanoparticles phytosynthesized using the extract on grapevine pathogens. The influence of some grapevine parameters (pith/wood rapport, soluble sugars, starch, total sugars, total water content, length of young shoots, number of grapes) in field experiments was also studied. The study was conducted on four clones (Feteasca alba 97 St., Feteasca neagra 6 St., Feteasca regala 72 St., and Cabernet Sauvignon 131 St.) located in vegetation pots inside a greenhouse. For the phytosynthesis of the silver nanoparticles (AgNPs) we used a scaled-up technology, allowing us to obtain large quantities of nanoparticles-containing solution. The AgNPs analysis by X-ray diffraction and transmission electron microscopy confirmed the synthesis of spherical and quasi-spherical nanoparticles of 17 nm average diameter and 6.72 nm crystallite size. The field experiments registered different responses of the four clones to the treatment, using both the natural extracts and phytosynthesized nanoparticles solution. Both recipes exhibited a protective effect against the Uncinula necator pathogen. For the treatment using phytosynthesized nanoparticles, significant increases in the pith/wood ratio for white wine clones (Feteasca alba 97 St. and Feteasca regala 72 St.) were observed. The biochemical analyses revealed other significant increases of soluble sugars (red wine clones-Feteasca neagra and Cabernet Sauvignon/second year), starch (Feteasca alba and Cabernet Sauvignon in 2021 for both clones), total sugars (Feteasca alba and Feteasca neagra in 2021 for both clones), and of total water content (Feteasca alba and Feteasca neagra in 2021 for both clones), respectively. The applied treatments also led to an increase of young shoots length and grape numbers for all clones as compared to the control (chemical pesticide), which would suggest a potential biostimulant effect of the recipes. |
