| Autor: |
Bunea MC; National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania., Diculescu VC; National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania., Enculescu M; National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania., Oprea D; National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania.; Faculty of Physics, University of Bucharest, Atomistilor 405, 077125 Magurele, Romania., Enache TA; National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania. |
| Abstrakt: |
Azathioprine (AZA) is a pharmacologic immunosuppressive agent administrated in various conditions such as autoimmune disease or to prevent the rejection of organ transplantation. The mechanism of action is based on its biologically active metabolite 6-mercaptopurine (6-MP), which is converted, among others, into thioguanine nucleotides capable of incorporating into replicating DNA, which may act as a strong UV chromophore and trigger DNA oxidation. The interaction between azathioprine and DNA, before and after exposure to solar simulator radiation, was investigated using UV-vis spectrometry and differential pulse voltammetry at a glassy carbon electrode. The results indicated that the interaction of AZA with UV radiation was pH-dependent and occurred with the formation of several metabolites, which induced oxidative damage in DNA, and the formation of DNA-metabolite adducts. Moreover, the viability assays obtained for the L929 cell culture showed that both azathioprine and degraded azathioprine induced a decrease in cell proliferation. |
