| Autor: |
Liang L; MQ Photonics Centre, Macquarie University, Sydney, NSW 2109, Australia., Everest-Dass AV; MQ Photonics Centre, Macquarie University, Sydney, NSW 2109, Australia., Kostyuk AB; Laboratory of Optical Theranostics, Nizhny Novgorod State University, 603950 Nizhny Novgorod, Russia., Khabir Z; MQ Photonics Centre, Macquarie University, Sydney, NSW 2109, Australia.; Australian Research Council Industrial Transformation Training Centre for Facilitated Advancement of Australia's Bioactives (FAAB), Macquarie University, Sydney, NSW 2109, Australia., Zhang R; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St. Lucia, QLD 4072, Australia., Trushina DB; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, 119333 Moscow, Russia., Zvyagin AV; MQ Photonics Centre, Macquarie University, Sydney, NSW 2109, Australia.; Laboratory of Optical Theranostics, Nizhny Novgorod State University, 603950 Nizhny Novgorod, Russia.; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia. |
| Abstrakt: |
Applications of nanoparticles (NPs) in the life sciences require control over their properties in protein-rich biological fluids, as an NP quickly acquires a layer of proteins on the surface, forming the so-called "protein corona" (PC). Understanding the composition and kinetics of the PC at the molecular level is of considerable importance for controlling NP interaction with cells. Here, we present a systematic study of hard PC formation on the surface of upconversion nanoparticles (UCNPs) coated with positively-charged polyethyleneimine (PEI) and negatively-charged poly (acrylic acid) (PAA) polymers in serum-supplemented cell culture medium. The rationale behind the choice of UCNP is two-fold: UCNP represents a convenient model of NP with a size ranging from 5 nm to >200 nm, while the unique photoluminescent properties of UCNP enable direct observation of the PC formation, which may provide new insight into this complex process. The non-linear optical properties of UCNP were utilised for direct observation of PC formation by means of fluorescence correlation spectroscopy. Our findings indicated that the charge of the surface polymer coating was the key factor for the formation of PC on UCNPs, with an ensuing effect on the NP-cell interactions. |
