| Autor: |
Seničar M; GLYcoDiag, 2 Rue du Cristal, 45100 Orléans, France., Roubinet B; GLYcoDiag, 2 Rue du Cristal, 45100 Orléans, France., Daniellou R; AgroParisTech, Cosmetology Department, 45100 Orléans, France., Prazuck T; Centre Hospitalier Régional d'Orléans, Servicedes Maladies Infectieuses et Tropicales, 45100 Orléans, France., Landemarre L; GLYcoDiag, 2 Rue du Cristal, 45100 Orléans, France. |
| Jazyk: |
angličtina |
| Zdroj: |
Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2022 Nov 18; Vol. 12 (11). Date of Electronic Publication: 2022 Nov 18. |
| DOI: |
10.3390/diagnostics12112860 |
| Abstrakt: |
Nasopharyngeal samples are currently accepted as the standard diagnostic samples for nucleic acid amplification testing and antigenic testing for the SARS-CoV-2 virus. In addition to the diagnostic capacity of SARS-CoV-2-positive crude nasopharyngeal samples, their qualitative potential for direct glycan-specific analysis, in order to uncover unique glycol profiles, was assessed. In this study we provide glycan characterization of SARS-CoV-2-positive and -negative nasopharyngeal samples directly from lectin interactions. Although with limited throughput, this study evaluated the clinical sensitivity and specificity of the GLYcoPROFILE® technology platformon45crude nasopharyngeal samples collected between November 2020 and April 2022. Each GLYcoPROFILE® of 39 SARS-CoV-2-positive samples was compared toglycoprofiling on a panel of 10 selected lectins and the results were paralleled with SARS-CoV-2-negative samples’ results. The GLYcoPROFILE® showed a clear distinction between positive and negative samples with WFA, GSL-II, PHA-L (GlcNAc-specific) and BPA (GalNAc-specific) highlighted as relevant lectins in SARS-CoV-2-positive samples. In addition, a significant, positive statistical correlation was found for these lectins (p < 0.01). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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