| Autor: |
Sprícigo JFW; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia 74690-900, Brazil., Guimarães ALS; Departamento de Medicina Veterinária, Centro Universitário Luterano de Palmas, CEULP, Palmas 77019-900, Brazil., Cunha ATM; Centro Universitário de Desenvolvimento do Centro Oeste, Luziânia 72852-580, Brazil.; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília 70910-900, Brazil., Leme LO; Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Reprodução Animal, Brasília 70770-917, Brazil., Carneiro MC; Escola de Veterinária e Zootecnia, Universidade Federal de Goiás, UFG, Goiânia 74690-900, Brazil., Franco MM; Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Reprodução Animal, Brasília 70770-917, Brazil.; Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia 38405-32, Brazil.; School of Veterinary Medicine, Federal University of Uberlândia, Uberlândia 38410-337, Brazil., Dode MAN; Programa de Pós-Graduação em Biologia Animal, Universidade de Brasília, Brasília 70910-900, Brazil.; Embrapa Recursos Genéticos e Biotecnologia, Laboratório de Reprodução Animal, Brasília 70770-917, Brazil. |
| Abstrakt: |
The present study aimed to determine whether cumulus cells (CC) biopsy, acquired before or after in vitro maturation (IVM), presents similar gene expression pattern and if would compromises oocyte quality. First, immature cumulus oocyte complexes (COCs) were distributed: (1) maturated in groups (control); (2) individually maturated, but not biopsied; (3) subjected to CC biopsy before maturation and individually matured; (4) individually matured and submitted to CC biopsy after maturation; (5) individually matured and CC biopsied before and after maturation. Secondly, candidate genes, described as potential markers of COCs quality, were quantified by RT-qPCR in CCs before and after IVM. After in vitro fertilization (IVF), zygotes were tracked and sorted regarding their developmental potential: fully developed to embryo, cleaved and arrested, and not-cleaved. The COC’s biopsy negatively affects embryo development (p < 0.05), blastocyst cell number (p < 0.05), and apoptotic cell ratio (p < 0.05), both before and after IVM. The PTGS2, LUM, ALCAM, FSHR, PGR, SERPINE2, HAS2, and PDRX3 genes were differentially expressed (p < 0.05) on matured CCs. Only PGR gene (p = 0.04) was under-expressed on matured CCs on Not-Cleaved group. The SERPINE2 gene was overexpressed (p = 0.01) in the Cleaved group on immature CCs. In summary, none of the selected gene studies can accurately predict COC’s fate after fertilization. |
