Acute restraint stress impairs histamine type 2 receptor ability to increase the excitability of medium spiny neurons in the nucleus accumbens.

Autor: Aceto G; Fondazione Policlinico Universitario Agostino Gemelli, IRCCS, Largo Agostino Gemelli 8, 00168 Roma, Italy; Department of Neuroscience, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy. Electronic address: giuseppe.aceto1@unicatt.it., Nardella L; Department of Neuroscience, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy. Electronic address: luca.nardella@unicatt.it., Lazzarino G; UniCamillus - Saint Camillus International University of Health and Medical Sciences, Via di Sant'Alessandro 8, 00131 Rome, Italy. Electronic address: giacomo.lazzarino@unicamillus.org., Tavazzi B; UniCamillus - Saint Camillus International University of Health and Medical Sciences, Via di Sant'Alessandro 8, 00131 Rome, Italy. Electronic address: barbara.Tavazzi@unicatt.it., Bertozzi A; Istituto di Analisi dei Sistemi ed Informatica 'Antonio Ruberti', National Research Council, Via dei Taurini 19, 00185 Roma, Italy. Electronic address: alessia.bertozzi@iasi.cnr.it., Nanni S; Fondazione Policlinico Universitario Agostino Gemelli, IRCCS, Largo Agostino Gemelli 8, 00168 Roma, Italy; Department of Translational Medicine and Surgery, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy. Electronic address: simona.nanni@unicatt.it., Colussi C; Fondazione Policlinico Universitario Agostino Gemelli, IRCCS, Largo Agostino Gemelli 8, 00168 Roma, Italy; Istituto di Analisi dei Sistemi ed Informatica 'Antonio Ruberti', National Research Council, Via dei Taurini 19, 00185 Roma, Italy. Electronic address: claudia.colussi@cnr.it., D'Ascenzo M; Fondazione Policlinico Universitario Agostino Gemelli, IRCCS, Largo Agostino Gemelli 8, 00168 Roma, Italy; Department of Neuroscience, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy. Electronic address: marcello.dascenzo@unicatt.it., Grassi C; Fondazione Policlinico Universitario Agostino Gemelli, IRCCS, Largo Agostino Gemelli 8, 00168 Roma, Italy; Department of Neuroscience, Università Cattolica del Sacro Cuore, Largo Francesco Vito 1, 00168 Rome, Italy. Electronic address: claudio.grassi@unicatt.it.
Jazyk: angličtina
Zdroj: Neurobiology of disease [Neurobiol Dis] 2022 Dec; Vol. 175, pp. 105932. Date of Electronic Publication: 2022 Nov 23.
DOI: 10.1016/j.nbd.2022.105932
Abstrakt: Histamine, a monoamine implicated in stress-related arousal states, is synthesized in neurons exclusively located in the hypothalamic tuberomammillary nucleus (TMN) from where they diffusely innervate striatal and mesolimbic networks including the nucleus accumbens (NAc), a vital node in the limbic loop. Since histamine-containing TMN neuron output increases during stress, we hypothesized that exposure of mice to acute restrain stress (ARS) recruits endogenous histamine type 2 receptor (H2R) signaling in the NAc, whose activation increases medium spiny neurons (MSNs) intrinsic excitability via downregulation of A-type K + currents. We employed an ARS paradigm in which mice were restrained for 120 min, followed by a 20-min recovery period, after which brain slices were prepared for ex vivo electrophysiology. Using whole-cell patch-clamp recordings, we found that pharmacological activation of H2R failed to affect MSN excitability and A-type K + currents in mice that underwent ARS. Interestingly, in mice treated with H2R-antagonist prior to ARS paradigm, H2R activation increased evoked firing and decreased A-type K + currents similarly to what observed in control mice. Furthermore, H2R-antagonist treatment ameliorated anxiety-like behavior in ARS mice. Together, our findings indicate that ARS paradigm recruits endogenous H2R signaling in MSNs and suggest the involvement of H2R signaling in stress-related motivational states.
Competing Interests: Declaration of competing interest All authors declare that the research was conducted in the absence of any commercial and financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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