Micro-vibration results in vitro-derived bovine blastocysts with greater cryotolerance, epigenetic abnormalities, and a massive transcriptional change.

Autor: Dos Santos AC; Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brazil., Joaquim DC; Vitrogen - Biotecnologia em Reprodução Animal, Cravinhos, SP, Brazil., Nociti RP; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil., Macabelli CH; Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brazil., Sampaio RV; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil; ST Genetics, Navasota, TX, USA., Oliveira AS; Vitrogen - Biotecnologia em Reprodução Animal, Cravinhos, SP, Brazil., Pita MO; WTA - Watanabe Tecnologia Aplicada, Cravinhos, SP, Brazil., de Oliveira RAM; WTA - Watanabe Tecnologia Aplicada, Cravinhos, SP, Brazil., da Silveira JC; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil., Meirelles FV; Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, SP, Brazil., Watanabe OY; WTA - Watanabe Tecnologia Aplicada, Cravinhos, SP, Brazil., Watanabe YF; Vitrogen - Biotecnologia em Reprodução Animal, Cravinhos, SP, Brazil., Chiaratti MR; Departamento de Genética e Evolução, Centro de Ciências Biológicas e da Saúde, Universidade Federal de São Carlos, São Carlos, SP, Brazil. Electronic address: marcos.chiaratti@ufscar.br.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2023 Jan 15; Vol. 196, pp. 214-226. Date of Electronic Publication: 2022 Nov 09.
DOI: 10.1016/j.theriogenology.2022.11.004
Abstrakt: Much effort has been employed to improve the quality of embryos obtained by in vitro production (IVP) given the relevance of this technology to current livestock systems. In this context, dynamic IVP systems have proved beneficial to the embryo once they mimic fluid flows and mechanical forces resulting from the movement of ciliated cells and muscle contraction in the reproductive tract. In the present study, we sought to confirm these initial findings as well as assess potential molecular consequences to the embryo by applying micro-vibration (45 Hz for 5 s once per 60 min) during both oocyte maturation and embryo culture in cattle. As a result, micro-vibration led to lower incidence of apoptosis in blastocysts following vitrification-thawing. Further analyses revealed epigenetic and transcriptional changes in blastocysts derived from the micro-vibration treatment, with a total of 502 differentially expressed genes. Enrichment analyses linked differentially expressed genes to 'Oxidative phosphorylation', 'Cytokine-cytokine receptor interaction', and 'Signaling pathways regulating pluripotency of stem cells'. Yet, a meta-analysis indicated that the transcriptional changes induced by micro-vibration were not toward that of in vivo-derived embryos. In conclusion, micro-vibration increases the cryoresistance of bovine embryos, but caution should be taken given the unclear consequences of epigenetic and transcriptional abnormalities induced by the treatment.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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