Pannier is a key regulator of embryogenesis, pupal development and female reproduction in the insect pest Bactrocera dorsalis.

Autor: Zhang Q; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Dou W; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., He LQ; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Yu SS; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Chen JQ; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Zheng LY; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Wang L; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China., Smagghe G; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China.; Department of Plants and Crops, Ghent University, Ghent, Belgium., Wang JJ; Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, China.; Academy of Agricultural Sciences, Southwest University, Chongqing, China.; International China-Belgium Joint Laboratory on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Jazyk: angličtina
Zdroj: Pest management science [Pest Manag Sci] 2023 Apr; Vol. 79 (4), pp. 1352-1361. Date of Electronic Publication: 2022 Dec 08.
DOI: 10.1002/ps.7305
Abstrakt: Background: Most arthropods are famous for their large reproductive capacity, with the ovary playing a vital role in the process. The study of the regulatory mechanisms of ovarian development may have the potential for a reproduction-based pest management strategy. GATA-binding transcription factors (GATAs) as important regulatory factors mediate many physiological processes, including development, immunity, insecticide resistance and reproduction. The Pannier (pnr), a member of GATA family, was confirmed to be involved in ovarian development of Bactrocera dorsalis in our previous study. However, the direct evidence of pnr regulating the fly ovarian development is still lacking.
Results: We used CRISPR/Cas9 to create Bdpnr loss-of-function mutations. Homozygous Bdpnr -/- mutants were nonviable, with most individuals dying during embryogenesis, some surviving to the larval stages, and the remaining few dying during pupation. In contrast, heterozygous individuals reached the adult stage, but ovarian development was disrupted, with concomitant decreases in egg laying and hatching rates. We also found that two genes encoding vitellogenin proteins (BdVg1 and BdVg2) and the vitellogenin receptor (BdVgR) were significantly down-regulated in heterozygous mutants compared to wild-type controls.
Conclusion: These results indicate that Bdpnr is required for embryonic and post-embryonic development, including the formation of ovaries. Bdpnr could therefore be considered as a molecular target for tephritid fly pest control. © 2022 Society of Chemical Industry.
(© 2022 Society of Chemical Industry.)
Databáze: MEDLINE
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