Plasmid sequence dataset of multidrug-resistant Enterobacterales isolated from hospital effluents and wastewater treatment plant.

Autor:	Mutuku C; Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, 7622 Pécs, Hungary., Kutasy B; Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences, Georgikon Campus Keszthely, 8360 Keszthely, Festetics Gy. u.7., Hungary., Urban P; Bioinformatics Research Group, Szentágothai Research Centre, 7624 Pécs, Hungary., Melegh S; Department of Medical Microbiology and Immunology, Medical School, University of Pécs, 7622, Pécs, Hungary., Herczeg R; Bioinformatics Research Group, Szentágothai Research Centre, 7624 Pécs, Hungary., Gazdag Z; Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, 7622 Pécs, Hungary., Virág E; Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, Egyetem Square 1, 4032 Debrecen, Hungary.
Jazyk:	angličtina
Zdroj:	Data in brief [Data Brief] 2022 Nov 09; Vol. 45, pp. 108736. Date of Electronic Publication: 2022 Nov 09 (Print Publication: 2022).
DOI:	10.1016/j.dib.2022.108736
Abstrakt:	We present plasmid sequences of 21 multidrug resistant isolates of Enterobacterales belonging to Escherichia coli (n=10), Klebsiella pneumoniae (n=9), Klebsiella oxytoca (n=1), and Citrobacter freundii (n=1). The isolates originated from effluent collected from hospital sewer pipes and from a wastewater treatment plant (WWTP) in a southwestern Hungarian city. Isolation was carried out using eosin methylene blue agar supplemented with ceftriaxone and the isolates were identified with MALDI-TOF MS. Screening for multidrug resistance was conducted by determining susceptibility to four chemical classes namely, beta-lactams, aminoglycoside, fluoroquinolone, and sulfonamide. Plasmid DNA was isolated by alkaline lysis method using the Monarch plasmid DNA miniprep kit from freshly grown pure colonies. Molecular typing and Illumina sequencing of plasmid DNA of multiresistant strains were performed. After the assembly of contigs, genes localized on plasmid sequences were determined and functionally annotated. These reconstructed plasmid sequences supplemented with gene functional annotations were deposited in the Mendeley data. Using these datasets different plasmid incompatibility groups were identified. These conjugative plasmids appear to play a key role in the transmission of multiple resistance genes in enteric bacteria via wastewater. The presented data may provide useful insight on the correlations between environmental antibiotic contamination and the development of bacterial resistance, which poses a serious public health threat. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2022 The Author(s).)
Databáze:	MEDLINE
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