Prevalence and geographic distribution of Babesia conradae and detection of Babesia vogeli in free-ranging California coyotes ( Canis latrans) .

Autor:	Javeed NN; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA., Shultz L; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA., Barnum S; Real-Time PCR Research and Diagnostics Core Facility, School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA 95616, USA., Foley JE; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA., Hodzic E; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA.; Real-Time PCR Research and Diagnostics Core Facility, School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA 95616, USA., Pascoe EL; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA., Martínez-López B; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA., Quinn N; South Coast Research and Extension Center, University of California, 1706 Irvine Blvd, Irvine CA, 92618, USA., Bucklin D; South Coast Research and Extension Center, University of California, 1706 Irvine Blvd, Irvine CA, 92618, USA., Dear JD; Department of Medicine and Epidemiology School of Veterinary Medicine, University of California Davis, 1 Garrod Dr, Davis, CA, 95616 USA.
Jazyk:	angličtina
Zdroj:	International journal for parasitology. Parasites and wildlife [Int J Parasitol Parasites Wildl] 2022 Nov 16; Vol. 19, pp. 294-300. Date of Electronic Publication: 2022 Nov 16 (Print Publication: 2022).
DOI:	10.1016/j.ijppaw.2022.11.004
Abstrakt:	Babesia species are intraerythrocytic piroplasms that can result in disease characterized by hemolytic anemia and thrombocytopenia. Of the 5 species that are known to infect canids in the United States, Babesia conradae is most frequently diagnosed in California, and Babesia vogeli is prevalent in the US. Despite the recent re-emergence of B. conradae , the mechanism of transmission is not known. Coyotes ( Canis latrans ) have been a proposed reservoir of disease, and previous work has shown that dogs with known aggressive interactions with coyotes are at greater risk for infection. This study aimed to determine the prevalence of B. conradae in wild coyote populations in California to assess the viability of coyotes as a potential source of infection for domestic dogs. Four hundred and sixty-one splenic samples were obtained during post-mortem examination of coyote carcasses from Southern California, Fresno, and Hopland. Demographic data including age, sex, cause of death, and urbanity were collected for each coyote. DNA was extracted from samples and amplified using real-time PCR with primers specific for the B. conradae ITS-2 gene. The 18S gene was amplified and sequenced using conventional PCR primers specific to the Babesia genus from any coyotes positive for B. conradae. In total, 22 coyotes tested positive for B. conradae in Fresno (n = 15), Orange (n = 4), San Bernardino (n = 1), and Los Angeles counties (n = 1) with an overall prevalence of 4.8%. Coyotes from Fresno ( P< .01) and rural coyotes ( P< .01) were significantly more likely to be infected with B. conradae . Ten of 14 samples sequenced were 99-100% homologous to B. conradae, and 4 samples were 100% homologous with B. vogeli DNA indicating co-infection with both pathogens. This study demonstrates that coyotes can become infected and harbor B. conradae and B. vogeli and should be investigated as a possible source of infection in domestic dogs. Competing Interests: None. (© 2022 The Authors.)
Databáze:	MEDLINE
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