Multi-color flow cytometer with PIC-based structured illumination.

Autor:	Tabatabaei Mashayekh A; Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany., Von Schoeler K; Miltenyi Biotec, Friedrich-Ebert-Straße 68, 51429 Bergisch Gladbach, Germany., Ackermann M; Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany., Klos T; TOPTICA Photonics AG, Lochhamer Schlag 19, 82166 Gräfelfing, Germany., Geuzebroek D; LioniX International BV, Hengelosestraat 500, 7521 Enschede, The Netherlands., Klein E; LioniX International BV, Hengelosestraat 500, 7521 Enschede, The Netherlands., Veenstra T; LioniX International BV, Hengelosestraat 500, 7521 Enschede, The Netherlands., Dekker R; LioniX International BV, Hengelosestraat 500, 7521 Enschede, The Netherlands., Merget F; Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany., Leisching P; TOPTICA Photonics AG, Lochhamer Schlag 19, 82166 Gräfelfing, Germany., Büscher M; Miltenyi Biotec, Friedrich-Ebert-Straße 68, 51429 Bergisch Gladbach, Germany., Witzens J; Institute of Integrated Photonics, RWTH Aachen University, Campus Blvd. 73, 52074 Aachen, Germany.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2022 Sep 06; Vol. 13 (10), pp. 5098-5115. Date of Electronic Publication: 2022 Sep 06 (Print Publication: 2022).
DOI:	10.1364/BOE.464903
Abstrakt:	We demonstrate a flow cytometer in which structured light illumination is used to attribute fluorescent and scattering signals to their excitation wavelength. A suitable multi-color light source emitting structured illumination patterns at 405, 488, 561 and 640 nm is developed based on a silicon nitride photonic integrated circuit and cytometry experiments are conducted with calibration beads. Performance metrics of the novel cytometer are compared with those of a mature, commercial device. While the experimental device still features a slightly higher sensitivity floor than the commercial one, all but the most weakly stained beads can be categorized. These promising results validate the feasibility of the proposed concept. Competing Interests: Miltenyi Biotec has a commercial interest in the developed cytometer. MB, KVS: Miltenyi Biotec (E), PL, TK: Toptica Photonics (E), DG, EK, TV, RD: LioniX International (E). (© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.)
Databáze:	MEDLINE
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