Biofilms and efflux pump regulatory gene ( mexR ) in multidrug-resistant Pseudomonas aeruginosa isolated from migratory birds in Egypt.

Autor: Elshafiee EA; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt., Khalefa HS; Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt., Al-Atfeehy NM; Biotechnology Unit, Reference Laboratory for Veterinary Quality Control on Poultry Production (RLQP), Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Nadi El-Seid St., P.O. Box 246, Dokki, Giza, 12618, Egypt., Amer F; Biotechnology Unit, Animal Health Research Institute, Animal Research Center, Nadi El-Seid St., P.O. Box 246, Dokki, Giza, 12618, Egypt., Hamza DA; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt., Ahmed ZS; Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, P.O. Box 12211, Giza, Egypt.
Jazyk: angličtina
Zdroj: Veterinary world [Vet World] 2022 Oct; Vol. 15 (10), pp. 2425-2431. Date of Electronic Publication: 2022 Oct 19.
DOI: 10.14202/vetworld.2022.2425-2431
Abstrakt: Background and Aim: Multidrug-resistant (MDR) Pseudomonas aeruginosa is a global threat to public health. This study aimed to determine biofilms and efflux pump regulatory gene ( mexR ) in MDR P. aeruginosa isolates.
Materials and Methods: A total of 42 fecal samples of aquatic migratory birds collected during hunting season in Egypt were evaluated for the detection of P. aeruginosa according to standard culture-based methods. The antibiotic susceptibility of P. aeruginosa strains was evaluated using disk diffusion methods. The biofilm formation ability of the isolates was phenotypically determined using a colorimetric microtitration plate assay. Polymerase chain reaction amplification was performed to detect biofilm genes ( PelA and PslA ) and mexR .
Results: In total, 19 isolates (45.2%) were recovered from the 42 fecal samples of migratory birds. All isolates were identified as MDR P. aeruginosa , and 78.9% of the strains produced biofilms at different degrees. Molecular detection of biofilm extracellular polymeric substances revealed that PelA was the most predominant gene in the biofilm-producing isolates, followed by PslA . mexR was detected in 63.2% of MDR P. aeruginosa isolates, and its prevalence was higher in non-biofilm-producing strains (75%) than in biofilm-producing strains (60%).
Conclusion: Antibiotic resistance in P. aeruginosa isolates recovered from migratory birds through various mechanisms is a major public and animal health problem. It is important to consider the significance of migratory birds in disease transmission.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © Elshafiee, et al.)
Databáze: MEDLINE
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