Sequential changes in cellular properties accompanying amniote somite formation.

Autor: Piatkowska AM; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK., Adhikari K; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK., Moverley AA; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK., Turmaine M; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK., Glazier JA; Department of Intelligent Systems Engineering, Biocomplexity Institute, Bloomington, Indiana, USA., Plachta N; Department of Cell and Developmental Biology, 9-123 Smilow Center for Translational Research, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA., Evans SE; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK., Stern CD; Department of Cell & Developmental Biology, University College London, Gower Street (Anatomy Building), London, UK.
Jazyk: angličtina
Zdroj: Journal of anatomy [J Anat] 2023 Mar; Vol. 242 (3), pp. 417-435. Date of Electronic Publication: 2022 Nov 24.
DOI: 10.1111/joa.13791
Abstrakt: Somites are transient structures derived from the pre-somitic mesoderm (PSM), involving mesenchyme-to-epithelial transition (MET) where the cells change their shape and polarize. Using Scanning electron microscopy (SEM), immunocytochemistry and confocal microscopy, we study the progression of these events along the tail-to-head axis of the embryo, which mirrors the progression of somitogenesis (younger cells located more caudally). SEM revealed that PSM epithelialization is a gradual process, which begins much earlier than previously thought, starting with the dorsalmost cells, then the medial ones, and then, simultaneously, the ventral and lateral cells, before a somite fully separates from the PSM. The core (internal) cells of the PSM and somites never epithelialize, which suggests that the core cells could be 'trapped' within the somitocoele after cells at the surfaces of the PSM undergo MET. Three-dimensional imaging of the distribution of the cell polarity markers PKCζ, PAR3, ZO1, the Golgi marker GM130 and the apical marker N-cadherin reveal that the pattern of polarization is distinctive for each marker and for each surface of the PSM, but the order of these events is not the same as the progression of cell elongation. These observations challenge some assumptions underlying existing models of somite formation.
(© 2022 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society.)
Databáze: MEDLINE
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