Distribution of Insecticide Resistance Genetic Markers in the West Nile Virus Vector Culex pipiens from South-Eastern Romania.

Autor: Stancu IG; Department of Genetics, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania.; Vector-Borne Infections Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Prioteasa FL; Medical Entomology Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Tiron GV; Vector-Borne Infections Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania.; Department of Microbiology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania., Cotar AI; Vector-Borne Infections Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Fălcuță E; Medical Entomology Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Porea D; Danube Delta National Institute for Research and Development, 165 Babadag, 820112 Tulcea, Romania., Dinu S; Molecular Epidemiology for Communicable Diseases Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Ceianu CS; Vector-Borne Infections Laboratory, Cantacuzino National Military Medical Institute for Research and Development, 103 Splaiul Independenței, 050096 Bucharest, Romania., Csutak O; Department of Genetics, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania.
Jazyk: angličtina
Zdroj: Insects [Insects] 2022 Nov 17; Vol. 13 (11). Date of Electronic Publication: 2022 Nov 17.
DOI: 10.3390/insects13111062
Abstrakt: Culex pipiens pipiens and Culex pipiens molestus mosquitoes are the vectors of West Nile virus in south-eastern Romania, an area of intense circulation and human transmission of this virus. The level of insecticide resistance for the mosquito populations in the region has not been previously assessed. Culex pipiens mosquitoes collected between 2018 and 2019 in south-eastern Romania from different habitats were subjected to biotype identification by real-time PCR. Substitutions causing resistance to organophosphates and carbamates (F290V and G119S in acetylcholinesterase 1) and to pyrethroids (L1014F in voltage gated Na + channel) were screened by PCR or sequencing. Substitutions F290V and G119S were detected at very low frequencies and only in heterozygous state in Culex pipiens molestus biotype specimens collected in urban areas. The molestus biotype population analysed was entirely homozygous for L1014F, and high frequencies of this substitution were also found for pipiens biotype and hybrid mosquitoes collected in urban and in intensive agriculture areas. Reducing the selective pressure by limiting the use of pyrethroid insecticides only for regions where it is absolutely necessary and monitoring L1014F mutation should be taken into consideration when implementing vector control strategies.
Databáze: MEDLINE
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