Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches.
| Autor: | Hadjidemetriou K; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, U.K., Kaur S; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, U.K., Cassidy CK; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, U.K., Zhang P; Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, U.K.; Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, U.K.; Chinese Academy of Medical Sciences Oxford Institute, University of Oxford, Oxford OX3 7BN, U.K. |
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| Jazyk: | angličtina |
| Zdroj: | Biochemical Society transactions [Biochem Soc Trans] 2022 Dec 16; Vol. 50 (6), pp. 1595-1605. |
| DOI: | 10.1042/BST20220191 |
| Abstrakt: | Chemotaxis signaling pathways enable bacteria to sense and respond to their chemical environment and, in some species, are critical for lifestyle processes such as biofilm formation and pathogenesis. The signal transduction underlying chemotaxis behavior is mediated by large, highly ordered protein complexes known as chemosensory arrays. For nearly two decades, cryo-electron tomography (cryoET) has been used to image chemosensory arrays, providing an increasingly detailed understanding of their structure and function. In this mini-review, we provide an overview of the use of cryoET to study chemosensory arrays, including imaging strategies, key results, and outstanding questions. We further discuss the application of molecular modeling and simulation techniques to complement structure determination efforts and provide insight into signaling mechanisms. We close the review with a brief outlook, highlighting promising future directions for the field. (© 2022 The Author(s).) |
| Databáze: | MEDLINE |
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