| Autor: |
Escalona-Villalpando RA; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico., Viveros-Palma K; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico., Espinosa-Lagunes FI; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Pedro Escobedo 76703, Mexico., Rodríguez-Morales JA; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico., Arriaga LG; Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Pedro Escobedo 76703, Mexico., Macazo FC; Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112, USA., Minteer SD; Department of Chemistry, University of Utah, 315 South 1400 East, Salt Lake City, UT 84112, USA., Ledesma-García J; División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Santiago de Querétaro 76010, Mexico. |
| Abstrakt: |
This work reports on Fe 2 O 3 and ZnO materials for lactate quantification. In the synthesis, the bi-phase γ-/α-Fe 2 O 3 and γ-/α-Fe 2 O 3 /ZnO nanoparticles (NPs) were obtained for their application in a lactate colorimetric sensor. The crystalline phases of the NPs were analyzed by XRD and XPS techniques. S/TEM images showed spheres with an 18 nm average and a needle length from 125 to 330 nm and 18 nm in diameter. The γ-/α-Fe 2 O 3 and γ-/α-Fe 2 O 3 /ZnO were used to evaluate the catalytic activity of peroxidase with the substrate 3,3,5,5-tetramethylbenzidine (TMB), obtaining a linear range of 50 to 1000 μM for both NPs, and a 4.3 μM and 9.4 μM limit of detection (LOD), respectively. Moreover, γ-/α-Fe 2 O 3 and γ-/α-Fe 2 O 3 /ZnO/lactate oxidase with TMB assays in the presence of lactate showed a linear range of 50 to 1000 µM, and both NPs proved to be highly selective in the presence of interferents. Finally, a sample of human serum was also tested, and the results were compared with a commercial lactometer. The use of ZnO with Fe 2 O 3 achieved a greater response toward lactate oxidation reaction, and has implementation in a lactate colorimetric sensor using materials that are economically accessible and easy to synthesize. |
