Development of a capillary electrophoretic method for determination of ketorolac enantiomers in human plasma using cationic β-cyclodextrin derivative as a chiral selector.

Autor: Kravchenko AV; Institute of Chemistry, The Department of Organic Chemistry, Saint Petersburg State University, Saint Petersburg, Russia., Kolobova EA; Institute of Chemistry, The Department of Organic Chemistry, Saint Petersburg State University, Saint Petersburg, Russia.; The Federal State Institute of Public Health 'The Nikiforov Russian Center of Emergency and Radiation Medicine', The Ministry of Russian Federation for Civil Defence, Emergencies and Elimination of Consequences of Natural Disasters, Saint Petersburg, Russia., Kechin AA; Institute of Chemistry, The Department of Organic Chemistry, Saint Petersburg State University, Saint Petersburg, Russia., Kartsova LA; Institute of Chemistry, The Department of Organic Chemistry, Saint Petersburg State University, Saint Petersburg, Russia.
Jazyk: angličtina
Zdroj: Journal of separation science [J Sep Sci] 2023 Jan; Vol. 46 (2), pp. e2200601. Date of Electronic Publication: 2022 Dec 11.
DOI: 10.1002/jssc.202200601
Abstrakt: A novel approach for the separation of ketorolac enantiomers by capillary electrophoresis is presented. A cationic β-cyclodextrin derivative based on imidazole was synthesized and used as a chiral selector in the background electrolyte. The influence of pH and ionic strength of background electrolyte, as well as cationic β-cyclodextrin derivative concentration on the resolution of ketorolac enantiomers, was investigated. The highest value of the resolution for ketorolac enantiomers was 1.46 when the background electrolyte consisted of 25 mM NaH 2 PO 4 (pH 6.4) with 1 mM 1-butyl-3-β-cyclodextrinimidazolium tosylate. Additionally, the possibilities of cationic derivatives for the separation of ketoprofen enantiomers were shown (peak resolution 1.06). The two-step preconcentration mode was developed to reduce the limit of detection of individual enantiomers. The proposed approach was successfully applied to determine ketorolac enantiomers in tablet "Ketorol express" and human plasma. The calibration range of ketorolac enantiomers for plasma samples was 0.25-2.50 μg/ml with coefficients of determination ≥ 0.99. The relative standard deviation both of the peak area and migration time was less than 15%, as well as the accuracy ranged from 90.1% to 110.2% for both analytes. The limits of detection were 44 and 55 ng/ml for R- and S-ketorolac. The quantity of ketorolac in plasma was verified with high-performance liquid chromatography.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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