Synthesis and Characterization of Eugenia uniflora L. Silver Nanoparticles and L-Cysteine Sensor Application.

Autor: Lopes IS; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Física, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil., Cassas F; Programa de Pós-Graduação em Biologia Química, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil., Veiga TAM; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Química, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil., de Oliveira Silva FR; Centro de Ciências e Tecnologia dos Materiais, Instituto de Pesquisas Energéticas e Nucleares, IPEN/CNEN-SP, SP, Brazil., Courrol LC; Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Departamento de Física, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 Jan; Vol. 20 (1), pp. e202200787. Date of Electronic Publication: 2022 Dec 13.
DOI: 10.1002/cbdv.202200787
Abstrakt: L-Cysteine (Cys) is a non-essential sulfur-containing amino acid, crucial for protein synthesis, detoxification, and several metabolic functions. Cys is widely used in the agricultural, food, cosmetic, and pharmaceutical industries. So, a suitable sensitive and selective sensing approach is of great interest, and a low-cost sensor would be necessary. This article presents silver nanoparticles (EuAgNPs) synthesized by a green synthesis method using Eugenia uniflora L. extracts and photoreduction. The nanoparticles were characterized by UV/VIS, transmission electron microscopy, high-performance liquid chromatography (HPLC), FTIR, and Zeta potential. With the addition of Cys in the EuAgNPs solution, the terminal thiol part of L-cysteine binds on the surface of nanoparticles through Ag-S bond. The EuAgNPs and CysAgNPs coexist until flavonoids bound the amino group of Cys, enhancing the red color of solutions. The EuAgNPs provided selectivity to detect Cys among other amino acids, and its detection limit was found to be 3.8 nM. The sensor has the advantages of low-cost synthesis, fast response, high selectivity, and sensitivity.
(© 2022 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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