Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion.

Autor: Meiers J; Chemical Biology of Carbohydrates (CBCH), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany.; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Department of Chemistry, Saarland University, 66123, Saarbrücken, Germany., Dastbaz J; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Department of Pharmacy, Saarland University, 66123, Saarbrücken, Germany.; Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany., Adam S; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Drug Design and Optimisation (DDOP), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany., Rasheed S; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Department of Pharmacy, Saarland University, 66123, Saarbrücken, Germany.; Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany., Kirsch SH; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany., Meiser P; URSAPHARM Arzneimittel GmbH, 66129, Saarbrücken, Germany., Gross P; Hochschule Kaiserslautern, Protein Chemistry Group, 66953, Pirmasens, Germany., Müller R; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Department of Pharmacy, Saarland University, 66123, Saarbrücken, Germany.; Microbial Natural Products (MINS), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany., Titz A; Chemical Biology of Carbohydrates (CBCH), Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research, 66123, Saarbrücken, Germany.; Deutsches Zentrum für Infektionsforschung (DZIF), Standort Hannover-Braunschweig, 38124, Braunschweig, Germany.; Department of Chemistry, Saarland University, 66123, Saarbrücken, Germany.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2023 Feb 01; Vol. 24 (3), pp. e202200463. Date of Electronic Publication: 2022 Dec 23.
DOI: 10.1002/cbic.202200463
Abstrakt: The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar K D in a carbohydrate-dependent fashion.
(© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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