Plate reader spectroscopy as an alternative to atomic absorption spectroscopy for the assessment of nanoparticle cellular uptake.

Autor:	Svitkova B; Department of Nanobiology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia., Selc M; Department of Nanobiology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.; Centre for Advanced Material Application, Slovak Academy of Sciences, Dubravska Cesta 9, 845 11 Bratislava, Slovakia., Nemethova V; Selecta Biotech SE, Istrijska 6094/20, 841 07 Bratislava, Slovakia., Razga F; Selecta Biotech SE, Istrijska 6094/20, 841 07 Bratislava, Slovakia., Gabelova A; Department of Nanobiology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia., Ursinyova M; Slovak Medical University, Limbova 12, 833 03 Bratislava, Slovakia., Babelova A; Department of Nanobiology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.; Centre for Advanced Material Application, Slovak Academy of Sciences, Dubravska Cesta 9, 845 11 Bratislava, Slovakia.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2022 Nov 16; Vol. 8 (11), pp. e11595. Date of Electronic Publication: 2022 Nov 16 (Print Publication: 2022).
DOI:	10.1016/j.heliyon.2022.e11595
Abstrakt:	Fundamental studies investigating the biological effects induced by nanoparticles (NPs) explicitly require the correct assessment of their intracellular concentration. Ultrasensitive atomic absorption spectroscopy (AAS) is perceived as one of the gold standard methods for quantifying internalized NPs. Besides its limitation to metal-based NPs though, AAS also requires specific infrastructure and tedious sample preparation and handling, making it time-consuming and cost-intensive. Herein we report on a reliable, rapid, and affordable alternative to AAS - plate reader spectroscopy (PRS), which offers an accessible option for everyday laboratory use without sophisticated instrumentation. Our results demonstrate, that following a proper methodological approach, data on intracellular concentration of NPs obtained by PRS are fully comparable to AAS results. Specifically, the intracellular concentration of magnetite NPs coated with sodium oleate and bovine serum albumin in human alveolar A549 cells was assessed by PRS and AAS in parallel, with a remarkable correlation coefficient of R = 0.9914. Competing Interests: The authors declare no conflict of interest. (© 2022 The Author(s).)
Databáze:	MEDLINE
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