Exposure to metal nanoparticles changes zeta potentials of Rhodococcus cells.
| Autor: | Kuyukina MS; Microbiology and Immunology Department, Perm State University, Perm, Russia.; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Russian Academy of Sciences, Perm, Russia., Makarova MV; Microbiology and Immunology Department, Perm State University, Perm, Russia., Pistsova ON; Microbiology and Immunology Department, Perm State University, Perm, Russia., Glebov GG; Microbiology and Immunology Department, Perm State University, Perm, Russia.; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Russian Academy of Sciences, Perm, Russia., Osipenko MA; Applied Mathematics and Mechanics Faculty, Perm National Research Polytechnic University, Perm, Russia., Ivshina IB; Microbiology and Immunology Department, Perm State University, Perm, Russia.; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Russian Academy of Sciences, Perm, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2022 Nov 15; Vol. 8 (11), pp. e11632. Date of Electronic Publication: 2022 Nov 15 (Print Publication: 2022). |
| DOI: | 10.1016/j.heliyon.2022.e11632 |
| Abstrakt: | Nanoparticles (NPs) of transition metals and their oxides are widely used in industries and exhibit diverse biological activities - from antimicrobial to growth promoting and regulating biofilms. In this study, the concentration-dependent effects of negatively charged metal and metal oxide NPs on the viability and net surface charge of Rhodococcus cells were revealed. Our hypothesis that zeta potential values of bacterial cells approach the zeta potential of NPs with an increase in the concentration of nanoparticles was statistically validated, thus suggesting the accumulation of nanoparticles on the cell surface. Thus, based on the dynamics of zeta potential, it would be possible to predict the accumulation of metal NPs on the cell surface of particular Rhodococcus species. It seemed that more toxic nanometals (e.g. CuO) accumulate more intensively on the bacterial cell wall than less toxic nanometals (Bi, Ni and Co). Physical properties of NPs, such as shape, size, dispersity and zeta potential, were characterized at different nanoparticle concentrations, in order to explain their diverse effects on bacterial viability, cellular charge and adhesion to hydrocarbons. Interestingly, an increase in Rhodococcus adhesion to n -hexadecane was observed in the presence of Cu and CuO NPs, while treatment with Fe Competing Interests: The authors declare no conflict of interest. (© 2022 The Authors.) |
| Databáze: | MEDLINE |
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