Expression analysis of Igs and mucosal immune responses upon SVCV infection in common carp ( Cyprinus carpio L. ).

Autor:	Wu S; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Meng K; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Wu Z; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Sun R; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Han G; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Qin D; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., He Y; Key Laboratory of Sichuan Province for Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, Sichuan 641100, China., Qin C; Key Laboratory of Sichuan Province for Conservation and Utilization of Fishes resources in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang, Sichuan 641100, China., Deng P; Wuhan Academy of Agricultural Sciences, Wuhan, Hubei 430207, China., Cao J; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Ji W; Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei 430070 China., Zhang L; Wuhan Academy of Agricultural Sciences, Wuhan, Hubei 430207, China., Xu Z; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, Hubei 430072, China.
Jazyk:	angličtina
Zdroj:	Fish and shellfish immunology reports [Fish Shellfish Immunol Rep] 2021 Dec 24; Vol. 3, pp. 100048. Date of Electronic Publication: 2021 Dec 24 (Print Publication: 2022).
DOI:	10.1016/j.fsirep.2021.100048
Abstrakt:	The immunoglobulin (Ig) is a crucial component of adaptive immune system in vertebrates including teleost fish. Here complete cDNA sequence of IgD heavy chain gene from common carp ( Cyprinus carpio ) was cloned and analyzed. The full-length cDNA of IgD heavy chain gene contained an open reading frame (ORF) of 2460 bp encoding 813 amino acids. According to amino acids sequence, multiple alignment and phylogenetic analysis showed that carp Igs are closely related to those of Cyprinidae fish. Transcriptional expression of IgD as well as IgM, IgZ1 and IgZ2 showed similar expression patterns in different organs, this is, high expression level in systemic immune tissues (ie, head kidney, heart and spleen) and low expression in mucosal tissues (ie, gill, skin and gut). Following viral infection with spring viraemia of carp virus (SVCV), obvious pathological changes in skin, gill and gut mucosa and up-regulated expression of antiviral related genes in skin, gill, gut and spleen were observed, indicating that SVCV successfully infected common carp and activated the systemic and mucosal immune system. Interestingly, IgM showed a significant up-regulation only in systemic tissue (spleen), but not in mucosal tissues (gut, gills and skin), while increased expression of IgZ1 and IgZ2 was found in gut. In contrast, the expression of IgD increased significantly in spleen, gills and skin. These strongly suggest that fish Ig isotypes play different roles in mucosal and systemic immunity during viral infection. Common carp (Cyprinus carpio); Igs; Spring viraemia of carp virus (SVCV). Competing Interests: None. (© 2021 The Author(s).)
Databáze:	MEDLINE
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