2-Hexadecenal Regulates ROS Production and Induces Apoptosis in Polymorphonuclear Leucocytes.

Autor: Semenkova GN; Department of Radiation Chemistry and Chemical-Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 4, Nezavisimosti av, 220030, Minsk, Belarus., Amaegberi NV; Department of Radiation Chemistry and Chemical-Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 4, Nezavisimosti av, 220030, Minsk, Belarus. amaegberi@bsu.by., Lisovskaya AG; Department of Radiation Chemistry and Chemical-Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 4, Nezavisimosti av, 220030, Minsk, Belarus.; Notre Dame Radiation Laboratory, University of Notre Dame, Notre Dame, IN, 46556, USA., Pinchuk SV; Institute of Biophysics and Cell Engineering of NAS of Belarus, 27, Academicheskaya st, 220072, Minsk, Belarus., Poleshko АG; Institute of Biophysics and Cell Engineering of NAS of Belarus, 27, Academicheskaya st, 220072, Minsk, Belarus., Shadyro OI; Department of Radiation Chemistry and Chemical-Pharmaceutical Technologies, Faculty of Chemistry, Belarusian State University, 4, Nezavisimosti av, 220030, Minsk, Belarus.; Research Institute for Physical and Chemical Problems, 14 Leningradskaya st., 220030, Minsk, Belarus.
Jazyk: angličtina
Zdroj: Cell biochemistry and biophysics [Cell Biochem Biophys] 2023 Mar; Vol. 81 (1), pp. 77-86. Date of Electronic Publication: 2022 Nov 23.
DOI: 10.1007/s12013-022-01117-w
Abstrakt: 2-Hexadecenal (2-HD)-a biologically active long-chain fatty aldehyde formed in organism enzymatically or nonenzymatically in the reaction of free-radical destruction of sphingolipids under the action of hypochlorous acid, producing by myeloperoxidase. This research aimed to study 2-HD effects on polymorphonuclear leukocytes' (PMNLs) functions. It has been shown that at submicromolar concentrations, 2-HD causes an elevation in ROS production by PMNLs. It has been found that such effect is associated with signal transduction pathways modification and expressed in elevation of NADPH oxidase, MPO, and JNK-MAPK contributions to this process. At higher concentrations, 2-HD induces apoptosis, which correlates with a significant increase in free Ca 2+ in the cytoplasm, a decrease in ROS production, and a decline in mitochondrial potential. Both of these processes are accompanied by cytoskeleton reorganization.
(© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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