An autoactive NB-LRR gene causes Rht13 dwarfism in wheat.

Autor: Borrill P; John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK., Mago R; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Xu T; Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK., Ford B; Grains Research and Development Corporation, Canberra, ACT 2600, Australia., Williams SJ; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Derkx A; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Bovill WD; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Hyles J; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Bhatt D; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Xia X; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., MacMillan C; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., White R; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia., Buss W; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Molnár I; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc 783 71 Czech Republic., Walkowiak S; Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3C 3G8, Canada.; University of Saskatchewan, Saskatoon, SK S7N 5A8 Canada., Olsen OA; Norwegian University of Life Sciences, Ås 1432, Norway., Doležel J; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of the Region Hana for Biotechnological and Agricultural Research, Olomouc 783 71 Czech Republic., Pozniak CJ; University of Saskatchewan, Saskatoon, SK S7N 5A8 Canada., Spielmeyer W; Commonwealth Scientific and Industrial Research Organisation (CSIRO) Agriculture and Food, Canberra, ACT 2601, Australia.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2022 Nov 29; Vol. 119 (48), pp. e2209875119. Date of Electronic Publication: 2022 Nov 23.
DOI: 10.1073/pnas.2209875119
Abstrakt: Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA)-insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA-sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here, we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat ( NB-LRR ) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable with Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional up-regulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodeling. Rht13 represents a new class of reduced height ( Rht ) gene, unlike other Rht genes, which encode components of the GA signaling or metabolic pathways. This discovery opens avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programs using a perfect genetic marker.
Databáze: MEDLINE
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