Bacterial species in the ruminal content of steers fed oilseeds in the diet.

Autor: de Melo HSA; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., Ítavo LCV; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil. luis.itavo@ufms.br., de Castro AP; Department of Biotechnology, Dom Bosco Catholic University, Av. Tamandaré, 6000, Jardim Seminário, Campo Grande, MS, CEP 79117-900, Brazil., Ítavo CCBF; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., de Araújo Caldas R; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., Mateus RG; Department of Biotechnology, Dom Bosco Catholic University, Av. Tamandaré, 6000, Jardim Seminário, Campo Grande, MS, CEP 79117-900, Brazil., Niwa MVG; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., de Moraes GJ; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., da Silva Zornitta C; College of Veterinary Medicine and Animal Science, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, MS, CEP 79070-900, Brazil., Gurgel ALC; Department of Animal Science, State University of Maringá, Maringá, PR, Brazil., Benchaar C; Sherbrooke Research and Development Centre, Agriculture and Agri-Food Canada, 2000 College Street, Sherbrooke, Quebec, Canada.
Jazyk: angličtina
Zdroj: Tropical animal health and production [Trop Anim Health Prod] 2022 Nov 23; Vol. 54 (6), pp. 396. Date of Electronic Publication: 2022 Nov 23.
DOI: 10.1007/s11250-022-03399-x
Abstrakt: The aim of this study was to evaluate bacterial species and diversity of methanogenic Archaea in the solid fraction of the ruminal content, through the gene sequences of the conserved 16S rDNA region, in response to the following diets: canola, cottonseed, sunflower, soybean, corn silage, and control diet. Six rumen-fistulated crossbred steers, with body weight (BW) of 416.33 ± 93.30 kg, were distributed in a 6 × 6 Latin square design. Regardless of the diet provided, amylolytic, proteolytic, and lactic bacteria were identified in the rumen fluid. Cellulolytic bacteria were predominant for all diets, reaching 47.75% of operational taxonomic units (OTUs) in animals fed with the cottonseed diet. Amylolytic bacteria reach 62.51% of OTU in animal fed sunflower diet, while proteolytic bacteria correspond to 65.96% of OTU in this same diet. Also, Megasphaera elsdenii bacterium was identified for all diets, with a greater percentage of OTU in steers fed the cottonseed diet. The diversity analysis of the species identified the methanogenic Archaea Methanobrevibacter ruminantium in all diets. We conclude that the control and corn silage diets have the most similar bacterial flora; diets with oilseeds had 47.5% similarity in rumen flora bacteria species. Animals fed with soybean showed a reduced number of methanogenic Archaea in the rumen content, which could be an alternative feed for cattle due to their low potential for energy losses with the production of methane.
(© 2022. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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