| Autor: |
Lopes KS; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil., Sousa HG; Federal University of Piauí, Campus Ministro Petrônio Portella, Teresina, Brazil., Artur E Silva Filho F; State University of Piauí, Campus Alexandre Alves Oliveira, Parnaíba, Brazil., da Silva Neta ER; Federal University of Piauí, Campus Ministro Petrônio Portella, Teresina, Brazil., de Lima SG; Federal University of Piauí, Campus Ministro Petrônio Portella, Teresina, Brazil., Dos Santos Rocha M; Federal University of Piauí, Campus Ministro Petrônio Portella, Teresina, Brazil., Marques RB; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil.; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil., da Costa CLS; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil., de Oliveira AN; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil., Bezerra DGP; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil., Alline Martins F; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil., de Almeida PM; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil.; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil., Uchôa VT; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil., Martins Maia Filho AL; Graduate Program in Chemistry/PPGQ-GERATEC-DQ - State University of Piauí, Teresina, Brazil.; Research Center in Biotechnology and Biodiversity - State University of Piauí, Teresina, Brazil. |
| Abstrakt: |
Croton heliotropiifolius Kunth, popularly known as "quince" and "velame," contains a high concentration of volatile oils in the leaves, and widely used in folk medicine as an antiseptic, analgesic, sedative, anti-inflammatory, spasmolytic and local anesthetic. The objectives of this investigation were to (1) identify the phytochemical compounds and (2) assess the cytogenotoxicity of the essential oil extracted from the leaves of C. heliotropiifolius Kunth. The oil was extracted utilizing hydrodistillation and phytochemical profile determined using gas chromatography and mass spectrometry (GCMS). In the toxicogenetics analysis, Allium cepa roots were exposed to 1% dimethylsulfoxide or methylmethanesulfonate (MMS, 10 µg/ml) negative and positive controls, respectively, and to C. heliotropiifolius oil at 6 concentrations (0.32; 1.6; 8; 40; 200 or 1000 µg/ml). The phytochemical profile exhibited 40 chromatographic bands, and 33 compounds identified. α-pinene (16.7%) and 1,8-cineole (13.81%) were identified as the major compounds. Some of these identified secondary metabolites displayed biological and pharmacological activities previously reported including antiseptic, analgesic, sedative, anti-inflammatory as well insecticidal, antiviral, anti-fungal actions. In the A. cepa test, C. heliotropiifolius leaves oil induced cytotoxicity at concentrations of 0.32, 1.6 or 200 µg/ml and genotoxicity at 200 or 1000 µg/ml as evidenced by increased presence of micronuclei and significant chromosomal losses. Based upon our observations data demonstrated that the essential oil of C. heliotropiifolius leaves contain monoterpene hydrocarbons, and oxygenated monoterpenes, sesquiterpenes, and oxygenated sesquiterpenes which are associated with cytotoxic and genotoxic responses noted in on A. cepa cells. |
