Evaluation of Receptor Activator of Nuclear Factor Kappa B Ligand, Osteoprotegerin, Osteopontin, and Tumor Necrosis Factor Alpha on Chronic Apical Periodontitis in Smokers.

Autor:	de Paula KM; Postgraduate Program in Dentistry, Dental Clinic Concentration Area, Department of Specific Formation, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., Gomes CC; Postgraduate Program in Dentistry, Dental Clinic Concentration Area, Department of Specific Formation, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil. Electronic address: cinthyagomes@id.uff.br., Valente MIB; Postgraduate Program in Dentistry, Dental Clinic Concentration Area, Department of Specific Formation, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., Pires FR; Postgraduate Program in Dentistry, Endodontics, Estácio de Sá University, Nova Friburgo, Rio de Janeiro, Brazil., Batistela Rodrigues Thuller KA; Postgraduate Program in Dentistry, Dental Clinic Concentration Area, Department of Specific Formation, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., Salles L; Postgraduate Program in Dentistry, Dental Clinic Concentration Area, Department of Specific Formation, Nova Friburgo Health Institute, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., Armada L; Postgraduate Program in Dentistry, Endodontics, Estácio de Sá University, Nova Friburgo, Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of endodontics [J Endod] 2023 Feb; Vol. 49 (2), pp. 137-143. Date of Electronic Publication: 2022 Nov 19.
DOI:	10.1016/j.joen.2022.11.012
Abstrakt:	Introduction: Smoking can be considered a risk factor for chronic apical periodontitis (CAP). This study compared the immunoexpression of biomarkers receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), osteopontin (OPN), and tumor necrosis factor alpha (TNF-α) in CAP in smokers and nonsmokers. Methods: Twelve smokers and 12 nonsmokers diagnosed with CAP and indicated for tooth extraction were selected. Exclusion factors were teeth with a diagnosis of root fracture, previous endodontic treatment, or endoperiodontal injury, in addition to individuals with systemic diseases, under 18 years of age, users of anti-inflammatory and/or antibiotics in the last 3 months, and drug users. Specimens were processed for histopathologic and immunohistochemical analysis. Results: Qualitative analysis of RANKL expression showed 66.66% weak/moderate and 33.33% strong in smokers and 100% weak/moderate in nonsmokers. OPG and OPN expressions were 100% negative to focal in the smoker group and 50% negative to focal and 50% weak/moderate in the nonsmoker group. TNF-α was 25% negative to focal and 75% weak/moderate in the smoker group and 33.33% negative to focal and 66.66% weak/moderate in the nonsmoker group. Quantitative analysis of the data using the Mann-Whitney U test showed that there was a significant difference in the immunoexpression of RANKL (P < .05), OPG (P < .05), and OPN (P < .05), but there was no statistical difference in the immunoexpression of TNF-α (P > .05) between the 2 groups. Conclusions: These findings suggest that smoking is capable of altering the inflammatory response, influencing the evolution of CAP. (Copyright © 2022 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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